QuantiChrom™ Sorbitol Dehydrogenase Assay Kit

Protocol SDS

Application

• For quantitative determination of sorbitol dehydrogenase enzyme activity.

Key Features

• Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 125 U/L for 12 min reaction.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological samples e.g. plasma, serum, urine, tissue, and culture media

Species

• All

Procedure

• 15 min

Size

• 100 tests

Detection Limit

• 0.1 U/L

Shelf Life

• 6 months

More Details

SORBITOL DEHYDROGENASE (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy. BioAssay Systems’ non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.