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QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit



Application

  • For quantitative colorimetric determination of glucose-6-phosphate concentration and evaluation of drug effects on its metabolism.

Key Features

  • Fast and sensitive. Linear detection range (20 µL sample): 0.2 to 100 U/L for 15 min reaction.
  • Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD565nm

Samples

  • Plasma, serum, tissue and culture media, etc

Species

  • All

Procedure

  • 20 min

Size

  • 100 tests

Detection Limit

  • 0.2 U/L

Shelf Life

  • 6 months

More Details

GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. BioAssay Systems’ non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in an NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

Glucose-6-Phosphate Dehydrogenase Kit
$439.00
- +

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

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