QuantiChrom™ β-Glucosidase Assay Kit
- For quantitative determination of β-glucosidase activity and evaluation of drug effects on its metabolism.
- High sensitivity and wide linear range. Use 20 µL sample. The detection limit is 2 U/L, linear up to 250 U/L.
- Homogeneous and simple procedure. Simple “mix-and-measure” procedure allows reliable quantitation of β-glucosidase activity within 20 minutes.
- Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments.
- 20 min
- 100 tests
- 2 U/L
- 6 months
More Detailsβ-GLUCOSIDASE is a glucosidase enzyme which acts upon β1->4 bonds linking two glucose or glucose-substituted molecules (i.e., the disaccharide cellobiose). β-Glucosidases are required by organisms (some fungi, bacteria, termites) for consumption of cellulose. Lysozyme is also a β-glucosidase and is present in tears to prevent bacterial infection of the eye. In humans, lower activity of a β-glucosidase isoform (lysosomal gluco-cerebrosidase) has been related to Gaucher’s disease and Parkinson’s disease. Simple, direct and automation-ready procedures for measuring β-glucosidase activity are becoming popular in Research and Drug Discovery. BioAssay Systems QuantiChrom™ β-Glucosidase Assay Kit is designed to measure β-glucosidase activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl-β-D-glucopyranoside that is hydrolyzed specifically by β-glucosidase into a yellow colored product (maximal absorbance at 405nm). The rate of the reaction is directly proportional to the enzyme activity.
How to use of DBGD-100 assay kit for human β-glucosidase activity?
The β-glucosidase assay comes with a neutal assay buffer (60 mM KPi, pH 7.0) and is suitable for enzymes that show activity around pH 7. The product of the reaction develops an intense yellow color at high pH (9-11).
The human enzyme has a pH optimum at 5.5. It is advisable to carry out the reaction in a buffer with that pH. However, because the absorbance of the colored product at pH 5 is lower than at pH 7, you have to raise the pH before measuring the absorbance.
Yang, W et al (2020). Increased α-synuclein oligomerization is associated with decreased activity of glucocerebrosidase in the aging human striatum and hippocampus. Neuroscience Letters, 733, 135093. Assay: β-glucosidase in human.
Yang, W et al (2020). α-Syn oligomers incubated with Parkinsons disease plasma promote neuron damage. International Journal of Clinical and Experimental Pathology, 13(8), 1995-2008. Assay: β-glucosidase in human plasma.
Moon, JY et al (2015). Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines. Animal Cells and Systems 19(5): 295-304. Assay: beta-Glucosidase in human cells.
Renchinkhand, G et al (2015). Identification of beta-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng. Journal of Food Biochemistry 40(1): 120-129. Assay: beta-Glucosidase in Enterococcus faecalis bacteria.
Liu, Z et al (2014). Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach. Biotechnology Letters 36(11): 2169-2176. Assay: beta-Glucosidase in mammal cells.
Mallery SR, et al (2011). Effects of human oral mucosal tissue, saliva, and oral microflora on intraoral metabolism and bioactivation of black raspberry anthocyanins. Cancer Prev Res (Phila). 4(8):1209-21. Assay: beta-Glucosidase in human saliva.
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