The β-glucosidase assay comes with a neutal assay buffer (60 mM KPi, pH 7.0) and is suitable for enzymes that show activity around pH 7. The product of the reaction develops an intense yellow color at high pH (9-11).
The human enzyme has a pH optimum at 5.5. It is advisable to carry out the reaction in a buffer with that pH. However, because the absorbance of the colored product at pH 5 is lower than at pH 7, you have to raise the pH before measuring the absorbance.
Yang, W et al (2020). Increased α-synuclein oligomerization is associated with decreased activity of glucocerebrosidase in the aging human striatum and hippocampus. Neuroscience Letters, 733, 135093. Assay: β-glucosidase in human.
Yang, W et al (2020). α-Syn oligomers incubated with Parkinsons disease plasma promote neuron damage. International Journal of Clinical and Experimental Pathology, 13(8), 1995-2008. Assay: β-glucosidase in human plasma.
Moon, JY et al (2015). Inhibition of cell growth and down-regulation of telomerase activity by amygdalin in human cancer cell lines. Animal Cells and Systems 19(5): 295-304. Assay: beta-Glucosidase in human cells.
Renchinkhand, G et al (2015). Identification of beta-Glucosidase Activity of Enterococcus Faecalis CRNB-A3 in Airag and Its Potential to Convert Ginsenoside Rb1 from Panax Ginseng. Journal of Food Biochemistry 40(1): 120-129. Assay: beta-Glucosidase in Enterococcus faecalis bacteria.
Liu, Z et al (2014). Mammalian expression levels of cellulase and xylanase genes optimised by human codon usage are not necessarily higher than those optimised by the extremely biased approach. Biotechnology Letters 36(11): 2169-2176. Assay: beta-Glucosidase in mammal cells.
Mallery SR, et al (2011). Effects of human oral mucosal tissue, saliva, and oral microflora on intraoral metabolism and bioactivation of black raspberry anthocyanins. Cancer Prev Res (Phila). 4(8):1209-21. Assay: beta-Glucosidase in human saliva.
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