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QuantiChrom™ Isocitrate Dehydrogenase Assay Kit



Application

  • For quantitative colorimetric kinetic determination of isocitrate dehydrogenase activity and evaluation of drug effects on its metabolism.

Key Features

  • Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 100 U/L for 30 min reaction.
  • Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD565nm

Samples

  • Plasma, serum, tissue and culture media, etc

Species

  • All

Procedure

  • 30 min

Size

  • 100 tests

Detection Limit

  • 0.1 U/L

Shelf Life

  • 6 months

More Details

ISOCITRATE DEHYDROGENASE (IDH) is an enzyme that catalyzes the interconversion of isocitrate and a-ketoglutarate. There are three IDH isoforms: IDH3 uses the cofactor NAD+ and catalyzes the third step in the citric acid cycle, while IDH1 and IDH2 use the cofactor NADP+ and catalyze the same reaction outside the citric acid cycle. This kit measures the activity of the NADP+ isoforms. Mutations in IDH1 and IDH2 have been linked with various brain tumors and acute myeloid leukemia. BioAssay Systems’ non-radioactive, colorimetric IDH assay is based on the reduction of the tetrazolium salt MTT in an NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.

Weight 1 lbs
Dimensions 9 × 6 × 5 in

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