QuantiChrom™ α-Mannosidase Assay Kit

Protocol SDS

Application

• For quantitative determination of α-mannosidase enzyme activity.

Key Features

• Fast and sensitive. Linear detection range (10 µL sample): 1 to 250 U/L for a 10-minute reaction.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD405nm

Samples

• Biological samples e.g. plasma, serum, tissue, and culture media

Species

• All

Procedure

• 10 min

Size

• 100 tests

Detection Limit

• 1 U/L

Shelf Life

• 6 months

More Details

α-MANNOSIDASE (AMA) is an enzyme that catalyzes the cleavage of the α form of mannose. α-Mannosidase assists in the breakdown of complex sugars from glycoproteins in the lysosome. Defective AMA or deficient AMA activity causes α-mannosidosis and leads to deterioration of the central nervous system in children. BioAssay Systems’ non-radioactive, colorimetric AMA assay is based on the cleavage of 4-nitrophenol from the synthetic substrate. Nitrophenol becomes intensely colored after the addition of the stop reagent. The increase in absorbance at 405 nm after the addition of the stop reagent is directly proportional to the enzyme activity.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.