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QuantiChrom™ Sorbitol Dehydrogenase Assay Kit
Application
- For quantitative determination of sorbitol dehydrogenase enzyme activity.
Key Features
- Fast and sensitive. Linear detection range (20 µL sample): 0.1 to 125 U/L for 12 min reaction.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD565nm
Samples
- Biological samples e.g. plasma, serum, urine, tissue and culture media
Species
- All
Procedure
- 15 min
Size
- 100 tests
Detection Limit
- 0.1 U/L
Shelf Life
- 6 months
More Details
- SORBITOL DEHYDROGENASE (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy. BioAssay Systems' non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
$459.00
For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
Why BioAssay Systems?
Technical support provided by the very scientists that develop the assays.
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Because we develop and manufacture the products, our prices are lower than competitors on the market
With over 200 different products, acquire all your assay kit needs in one order.
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