QuantiChrom™ Pectinase Assay Kit

Protocol SDS

Application

• For quantitative determination of pectinase activity determination in biological samples.

Key Features

• Linear detection range from 9.2-100 U/L pectinase activity in a 96-well plate assay.
• Simple and convenient 40-minute “Add-Mix-Measure” type assay. High-throughput format and compatible with laboratory liquid handling systems. No heating is required.
• The kit does not use toxic materials. Refer to the previously established DNS method.

Method

• OD600nm

Samples

• Enzyme extracts, agriculture, and biological samples

Species

• Plant, fungal tissues, juice, bacteria, etc

Procedure

• 40 min

Size

• 100 tests

Detection Limit

• 9.2 U/L

Shelf Life

• 12 months

More Details

PECTINASE (EC 3.2.1.15) is an enzyme that catalyzes the hydrolysis of pectin, a heteropolysaccharide found primarily in the middle lamella and cell wall of terrestrial plants. During fruit ripening, enzymes, including pectinase break down the middle lamella, separating cells and softening the fruit. Pectinase enzymes are used in the food industry to liquefy fruit pulp and clarify fruit juice and wine. BioAssay Systems’ DPEC-100 kit provides a rapid and convenient assay for pectinase enzyme activity, in which pectin the substrate forms a turbid complex with the detection reagent. The turbidity, measured at OD600nm, is proportional to the amount of unhydrolyzed pectin, thus inversely proportional to the pectinase activity.

Will pectin lyase work with this kit?

Pectin lyase (EC 4.2.2.10) and other enzymes that hydrolyze pectin will likely work with this kit, however the enzyme unit definition for this kit refers to endopolygalacturonase (EC 3.2.1.15). So the results of this kit are reported as activity of EC 3.2.1.15.

Why is there no pectinase enzyme standard in this kit?

Pectinase may not remain stable so pectinase activity is matched with the included calibrator. The optical density of the calibrator matches the optical density of wells assayed with 100 U/L pectinase under the standard assay conditions.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.