QuantiChrom™ ATPase Assay Kit

Application

• For quantitative determination of ATPase or GTPase activity and high-throughput screen for their inhibitors.

Key Features

• High sensitivity: detection of 0.007 U/L ATPase or GTPase activity.
• Fast and convenient: single reagent, homogeneous “mix-and-measure” assay allows quantitation of enzyme activity within 30 minutes.
• Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Method

• OD620nm

Samples

• Compounds that affect ATPase/GTPase activity

Species

• All

Procedure

• 30 min

Size

• 200 tests

Detection Limit

• 0.007 U/L

Shelf Life

• 12 months

More Details

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

De Hita, D et al. (2020). Discriminating the short-term action of root and foliar application of humic acids on plant growth: Emerging role of jasmonic acid. Frontiers in Plant Science, 11, 493. Assay: ATPase Assay Kit in cucumber roots.

Gong, D. et al. (2018). Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin. Nature communications 9(1):3623. Assay: GTPase in human hPMCA1-NPTN and hPMCA1.

Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T., & Kimura, S. (2018). Aciduricity and acid tolerance mechanisms of Streptococcus anginosus. The Journal of general and applied microbiology, 2017-11. Assay: GTPase in Streptococcus anginosus NCTC 10713.

Xu, X., Ji, J., Xu, Q., Qi, X., Weng, Y., & Chen, X. (2018). The major-effect quantitative trait locus Cs ARN 6.1 encodes an AAA ATP ase domain-containing protein that is associated with waterlogging stress tolerance by promoting adventitious root formation. The Plant Journal, 93(5), 917-930. Assay: GTPase in E. coli pCzn1-460 vector.

Yang, X., Chen, C., Tian, H., Chi, H., Mu, Z., Zhang, T. & Ji, X. (2018). Mechanism of ATP hydrolysis by the Zika virus helicase. The FASEB Journal, 32(10), 5250-5257. Assay: GTPase in enzymes.

Zhou, J. et al. (2018). Porcine Mx1 protein inhibits classical swine fever virus replication by targeting nonstructural protein NS5B. Journal of virology, 92(7), e02147-17. Assay: GTPase in porcine PK-15 cells.

Chen, K., Guo, L., Zhang, J., Chen, Q., Wang, K., Li, C. & Chen, D. (2017). A gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1. Acta biomaterialia, 48, 215-226. Assay: GTPase in mice RanGAP1.

Qian, H., Zhao, X., Cao, P., Lei, J., Yan, N., & Gong, X. (2017). Structure of the human lipid exporter ABCA1. Cell, 169(7), 1228-1239. Assay: GTPase in human ABCA1.

Sheng, Y., Song, Y., Li, Z., Wang, Y., Lin, H., Cheng, H., & Zhou, R. (2017). RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly. Cell Death & Differentiation 25(5):918-934. Assay: GTPase in COS-7 and HeLa cells RAB37.

Sun, S. et al. (2017). Cryo-EM structures of the ATP-bound Vps4 E233Q hexamer and its complex with Vta1 at near-atomic resolution. Nature communications, 8, 16064. Assay: GTPase in Saccharomyces cerevisiae Vps4 proteins.

Ayares, D. (2016). Multi-transgenic pigs for diabetes treatment. U.S. Patent No. 9,339,519. Assay: GTPase in porcine fetal fibroblasts.

Cao, X., Li, Y., Jin, X., Li, Y., Guo, F., & Jin, T. (2016). Molecular mechanism of divalent-metal-induced activation of NS3 helicase and insights into Zika virus inhibitor design. Nucleic acids research 44(21):10505-10514. Assay: GTPase in ZIKV NS3 helicase.

Cho, J. H., Lee, J. H., Park, Y. K., Choi, M. N., & Kim, K. N. (2016). Calcineurin B-like protein CBL10 directly interacts with TOC34 (Translocon of the Outer membrane of the Chloroplasts) and decreases its GTPase activity in Arabidopsis. Frontiers in Plant Science 7, 1911. Assay: GTPase in E. coli TOC34 protein.

El-Shesheny, I., Hijaz, F., El-Hawary, I., Mesbah, I., & Killiny, N. (2016). Impact of different temperatures on survival and energy metabolism in the Asian citrus psyllid, Diaphorina citri Kuwayama. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 192, 28-37. Assay: GTPase in Diaphorina citri hsp 70.

Killiny, N., Hijaz, F., Ebert, T. A., & Rogers, M. E. (2016). Plant bacterial pathogen manipulates the energy metabolism of its insect vector. Applied and Environmental Microbiology 83(5). pii: e03005-16. Assay: GTPase in Diaphorina citri.

Seyedmohammad, S., Fuentealba, N. A., Marriott, R. A., Goetze, T. A., Edwardson, J. M., Barrera, N. P., & Venter, H. (2016). Structural model of FeoB, the iron transporter from Pseudomonas aeruginosa, predicts a cysteine lined, GTP-gated pore. Bioscience reports, 36(2), e00322. Assay: GTPase in Pseudomonas aeruginosa FeoB Transporter.

Tian, H. et al. (2016). Structural basis of Zika virus helicase in recognizing its substrates. Protein & cell, 7(8), 562-570. Assay: GTPase in ZIKV helicase172-617.

Yang, J. et al (2016). Pironetin reacts covalently with cysteine-316 of alpha-tubulin to destabilize microtubule. Nature communications, 7, 12103. Assay: GTPase in E. coli tubulin.

Bertol JW, et al (2011). Antiherpes activity of glucoevatromonoside, a cardenolide isolated from a Brazilian cultivar of Digitalis lanata. Antiviral Res. 92(1):73-80. Assay: ATPase/GTPase in human, monkey Na(+)K(+)ATPase.

Binder M, et al (2011). Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I). J Biol Chem. 286(31):27278-87. Assay: ATPase/GTPase in human purified protein.

Hussey, GS et al (2011). Identification of an mRNP complex regulating tumorigenesis at the translational elongation step. Mol Cell. 41(4):419-31. Assay: ATPase/GTPase in human GTPase.

Sheikha GA, et al (2011). Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation. J Enzyme Inhib Med Chem. 26.5: 603-609. Assay: ATPase/GTPase in human Hsp90 inhibition.

Sheikha, GA et al (2010). Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation. J Enzyme Inhib Med Chem. 2010 Dec 29. [Epub ahead of print]. Assay: ATPase/GTPase in human Hsp90 inhibition.

Suematsu T, et al (2010). A bacterial elongation factor G homologue exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi. Mol Microbiol. 75(6):1445-54. Assay: ATPase/GTPase in bacteria/human ATPase.

Hu WW, et al (2009). Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces. J Control Release. 135(3):250-8. Assay: ATPase/GTPase in ATPase extract.

Gong, D. et al. (2018). Structure of the human plasma membrane Ca2+-ATPase 1 in complex with its obligatory subunit neuroplastin. Nature communications 9(1):3623. Assay: GTPase in human hPMCA1-NPTN and hPMCA1.

Sasaki, M., Kodama, Y., Shimoyama, Y., Ishikawa, T., & Kimura, S. (2018). Aciduricity and acid tolerance mechanisms of Streptococcus anginosus. The Journal of general and applied microbiology, 2017-11. Assay: GTPase in Streptococcus anginosus NCTC 10713.

Xu, X., Ji, J., Xu, Q., Qi, X., Weng, Y., & Chen, X. (2018). The major-effect quantitative trait locus Cs ARN 6.1 encodes an AAA ATP ase domain-containing protein that is associated with waterlogging stress tolerance by promoting adventitious root formation. The Plant Journal, 93(5), 917-930. Assay: GTPase in E. coli pCzn1-460 vector.

Yang, X., Chen, C., Tian, H., Chi, H., Mu, Z., Zhang, T. & Ji, X. (2018). Mechanism of ATP hydrolysis by the Zika virus helicase. The FASEB Journal, 32(10), 5250-5257. Assay: GTPase in enzymes.

Zhou, J. et al. (2018). Porcine Mx1 protein inhibits classical swine fever virus replication by targeting nonstructural protein NS5B. Journal of virology, 92(7), e02147-17. Assay: GTPase in porcine PK-15 cells.

Chen, K., Guo, L., Zhang, J., Chen, Q., Wang, K., Li, C. & Chen, D. (2017). A gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1. Acta biomaterialia, 48, 215-226. Assay: GTPase in mice RanGAP1.

Qian, H., Zhao, X., Cao, P., Lei, J., Yan, N., & Gong, X. (2017). Structure of the human lipid exporter ABCA1. Cell, 169(7), 1228-1239. Assay: GTPase in human ABCA1.

Sheng, Y., Song, Y., Li, Z., Wang, Y., Lin, H., Cheng, H., & Zhou, R. (2017). RAB37 interacts directly with ATG5 and promotes autophagosome formation via regulating ATG5-12-16 complex assembly. Cell Death & Differentiation 25(5):918-934. Assay: GTPase in COS-7 and HeLa cells RAB37.

Sun, S. et al. (2017). Cryo-EM structures of the ATP-bound Vps4 E233Q hexamer and its complex with Vta1 at near-atomic resolution. Nature communications, 8, 16064. Assay: GTPase in Saccharomyces cerevisiae Vps4 proteins.

Ayares, D. (2016). Multi-transgenic pigs for diabetes treatment. U.S. Patent No. 9,339,519. Assay: GTPase in porcine fetal fibroblasts.

Cao, X., Li, Y., Jin, X., Li, Y., Guo, F., & Jin, T. (2016). Molecular mechanism of divalent-metal-induced activation of NS3 helicase and insights into Zika virus inhibitor design. Nucleic acids research 44(21):10505-10514. Assay: GTPase in ZIKV NS3 helicase.

Cho, J. H., Lee, J. H., Park, Y. K., Choi, M. N., & Kim, K. N. (2016). Calcineurin B-like protein CBL10 directly interacts with TOC34 (Translocon of the Outer membrane of the Chloroplasts) and decreases its GTPase activity in Arabidopsis. Frontiers in Plant Science 7, 1911. Assay: GTPase in E. coli TOC34 protein.

El-Shesheny, I., Hijaz, F., El-Hawary, I., Mesbah, I., & Killiny, N. (2016). Impact of different temperatures on survival and energy metabolism in the Asian citrus psyllid, Diaphorina citri Kuwayama. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 192, 28-37. Assay: GTPase in Diaphorina citri hsp 70.

Killiny, N., Hijaz, F., Ebert, T. A., & Rogers, M. E. (2016). Plant bacterial pathogen manipulates the energy metabolism of its insect vector. Applied and Environmental Microbiology 83(5). pii: e03005-16. Assay: GTPase in Diaphorina citri.

Seyedmohammad, S., Fuentealba, N. A., Marriott, R. A., Goetze, T. A., Edwardson, J. M., Barrera, N. P., & Venter, H. (2016). Structural model of FeoB, the iron transporter from Pseudomonas aeruginosa, predicts a cysteine lined, GTP-gated pore. Bioscience reports, 36(2), e00322. Assay: GTPase in Pseudomonas aeruginosa FeoB Transporter.

Tian, H. et al. (2016). Structural basis of Zika virus helicase in recognizing its substrates. Protein & cell, 7(8), 562-570. Assay: GTPase in ZIKV helicase172-617.

Yang, J. et al (2016). Pironetin reacts covalently with cysteine-316 of alpha-tubulin to destabilize microtubule. Nature communications, 7, 12103. Assay: GTPase in E. coli tubulin.

Bertol JW, et al (2011). Antiherpes activity of glucoevatromonoside, a cardenolide isolated from a Brazilian cultivar of Digitalis lanata. Antiviral Res. 92(1):73-80. Assay: ATPase/GTPase in human, monkey Na(+)K(+)ATPase.

Binder M, et al (2011). Molecular Mechanism of Signal Perception and Integration by the Innate Immune Sensor Retinoic Acid-inducible Gene-I (RIG-I). J Biol Chem. 286(31):27278-87. Assay: ATPase/GTPase in human purified protein.

Hussey, GS et al (2011). Identification of an mRNP complex regulating tumorigenesis at the translational elongation step. Mol Cell. 41(4):419-31. Assay: ATPase/GTPase in human GTPase.

Sheikha GA, et al (2011). Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation. J Enzyme Inhib Med Chem. 26.5: 603-609. Assay: ATPase/GTPase in human Hsp90 inhibition.

Sheikha, GA et al (2010). Some sulfonamide drugs inhibit ATPase activity of heat shock protein 90: investigation by docking simulation and experimental validation. J Enzyme Inhib Med Chem. 2010 Dec 29. [Epub ahead of print]. Assay: ATPase/GTPase in human Hsp90 inhibition.

Suematsu T, et al (2010). A bacterial elongation factor G homologue exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi. Mol Microbiol. 75(6):1445-54. Assay: ATPase/GTPase in bacteria/human ATPase.

Hu WW, et al (2009). Digoxigenin modification of adenovirus to spatially control gene delivery from chitosan surfaces. J Control Release. 135(3):250-8. Assay: ATPase/GTPase in ATPase extract.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

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