Yes, this kit works for urine of all species.
Yes, urine varies widely from individual to individual. Depending on the concentration of the urine and the diet of the individual, each sample will respond to the reagent slightly differently. The internal standard corrects for the slight differences thus calculating accurate results.
No, you only need to run a blank in duplicate for each assay. For example, if you are running 5 samples, you will have 10 internal standard wells, 10 sample wells, and 2 blank wells.
Yes, unused reagents can be stored in the refridgerator until next use. Be sure to equilibrate reagents to room temperature before using again.
For assays that use an internal standard, running a standard for each sample would be cumbersome, and would consume large quantities of reagents. It is standard practice to use a one point standard to calculate your values.
Perez Gutierrez, R. M., Garcia Campoy, A. H., Paredes Carrera, S. P., Muniz Ramirez, A., Mota Flores, J. M., Valle, F., & Odin, S. (2019). 3′-O-beta-d-glucopyranosyl-alpha, 4, 2′, 4′, 6′-pentahydroxy-dihydrochalcone, from Bark of Eysenhardtia polystachya Prevents Diabetic Nephropathy via Inhibiting Protein Glycation in STZ-Nicotinamide Induced Diabetic Mice. Molecules, 24(7), 1214. Assay: Protein creatinine ratio in mice serum.
Xie, P., Young, M. W., Bian, H., Niknam-Bienia, S., Hong, S., Mustoe, T. A., & Galiano, R. D. (2019). Renal dysfunction aggravated impaired cutaneous wound healing in diabetic mice. Wound Repair and Regeneration, 27(1), 49-58. Assay: Protein creatinine ratio in mice blood.
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