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QuantiChrom™ Phytase Assay Kit



Application

  • A simplest, direct “Add-and-Measure” assay for phytase enzyme activity in agricultural and biological samples.

Key Features

  • Simple and convenient: No complex detection reagents to mix.
  • High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay.
  • Fast and high-throughput: Homogeneous “mix-and-measure” assay allows for quantification of phytase activity within 60 minutes.

Method

  • OD620nm

Samples

  • Enzyme extracts, agriculture and biological samples

Species

  • All (e.g. Wheat, Barley)

Procedure

  • 60 min

Size

  • 100 tests

Detection Limit

  • 0.01 U/L

Shelf Life

  • 12 months

More Details

  • PHYTASE catalyzes the hydrolysis of phosphoester bonds on myoinositol-(1,2,3,4,5,6)-hexakisphosphate (Phytic acid or IP6) thereby releasing inositol and phosphate. Phytic acid is a major storage reservoir for phosphate in plants. Phytase is abundant in grains such as wheat and barley, and the hydrolysis of phytic acid by single-stomached animals is a crucial aspect of animal nutrition. In addition, the lack of phytase in single-stomached animals can lead to excessive phosphorus leaching into the environment due to undigested phytic acid.

    Simple, direct and automation-ready procedures for measuring phytase activity are very desirable. BioAssay Systems’ QuantiChromTM Phytase Assay is based on our Malachite Green Phosphate Assay (POMG-25H). The color intensity, measured at 620 nm, is proportionate to the amount of phosphate released from phytic acid.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.

$399.00

For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: 4°C upon receipt

Why BioAssay Systems?

Technical support provided by the very scientists that develop the assays.

Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Because we develop and manufacture the products, our prices are lower than competitors on the market

With over 200 different products, acquire all your assay kit needs in one order.

Products used by clients worldwide with distributors in over 60 countries.