A simplest, direct “Add-and-Measure” assay for phytase enzyme activity in agricultural and biological samples.
Key Features
Simple and convenient: No complex detection reagents to mix.
High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay.
Fast and high-throughput: Homogeneous “mix-and-measure” assay allows for quantification of phytase activity within 60 minutes.
Method
OD620nm
Samples
Enzyme extracts, agriculture, and biological samples
Species
All (e.g. Wheat, Barley)
Procedure
60 min
Size
100 tests
Detection Limit
0.01 U/L
Shelf Life
12 months
More Details
PHYTASE catalyzes the hydrolysis of phosphoester bonds on myoinositol-(1,2,3,4,5,6)-hexakisphosphate (Phytic acid or IP6) thereby releasing inositol and phosphate. Phytic acid is a major storage reservoir for phosphate in plants. Phytase is abundant in grains such as wheat and barley, and the hydrolysis of phytic acid by single-stomached animals is a crucial aspect of animal nutrition. In addition, the lack of phytase in single-stomached animals can lead to excessive phosphorus leaching into the environment due to undigested phytic acid.
Simple, direct, and automation-ready procedures for measuring phytase activity are very desirable. BioAssay Systems’ QuantiChromTM Phytase Assay is based on our Malachite Green Phosphate Assay (POMG-25H). The color intensity, measured at 620 nm, is proportionate to the amount of phosphate released from phytic acid.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
