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QuantiChrom™ Phytase Assay Kit
Application
- A simplest, direct “Add-and-Measure” assay for phytase enzyme activity in agricultural and biological samples.
Key Features
- Simple and convenient: No complex detection reagents to mix.
- High sensitivity and wide detection range: Detection range of 0.01 to 20 U/L phytase in a 96-well plate assay.
- Fast and high-throughput: Homogeneous “mix-and-measure” assay allows for quantification of phytase activity within 60 minutes.
Method
- OD620nm
Samples
- Enzyme extracts, agriculture, and biological samples
Species
- All (e.g. Wheat, Barley)
Procedure
- 60 min
Size
- 100 tests
Detection Limit
- 0.01 U/L
Shelf Life
- 12 months
More Details
PHYTASE catalyzes the hydrolysis of phosphoester bonds on myoinositol-(1,2,3,4,5,6)-hexakisphosphate (Phytic acid or IP6) thereby releasing inositol and phosphate. Phytic acid is a major storage reservoir for phosphate in plants. Phytase is abundant in grains such as wheat and barley, and the hydrolysis of phytic acid by single-stomached animals is a crucial aspect of animal nutrition. In addition, the lack of phytase in single-stomached animals can lead to excessive phosphorus leaching into the environment due to undigested phytic acid.Simple, direct, and automation-ready procedures for measuring phytase activity are very desirable. BioAssay Systems’ QuantiChromTM Phytase Assay is based on our Malachite Green Phosphate Assay (POMG-25H). The color intensity, measured at 620 nm, is proportionate to the amount of phosphate released from phytic acid.No frequently asked questions for this new product. Please check back later.
For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$419.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: 4°C upon receipt
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