Shopping Cart

EnzyFluo™ NFKB Phosphorylation Assay Kit



Application

  • For quantitative fluorescent immunoenzymatic assay of NFkB phosphorylation status in cultured cells.

Key Features

  • New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).
  • Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis is necessary.
  • Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

  • Cell-Based ELISA (FL530/585 nm, 360/450 nm)

Samples

  • Cells

Species

  • Human, mouse

Procedure

  • The assay takes 6.5 hrs, hands-on time 2.5 hrs

Size

  • 100 tests

Shelf Life

  • 6 months

More Details

  • Nuclear factor-kappa B (NFkB) is a transcription factor that plays a central role in many physiological processes, e.g. inflammation, tumorigenesis, and apoptosis. NFkB is activated by a wide variety of stimuli, including inflammatory cytokines such as TNF-α. NFkB is a dimer composed of members of the Rel family of proteins: p65/RelA, c-Rel, RelB, NFkB1/p50, and NFkB2/p52. Phosphorylation of p65/RelA at Ser-536 results in decreased nuclear export and enhanced p65/RelA-dependent transcription. BioAssay Systems cell-based ELISA measures phosphorylated p65(S536) (pNFkB) in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study NFkB regulation in short-term and long-term assays.

    Haven’t Found the ELISA Kit for Your Target


    We offer two DIY kits: Custom Cell-based ELISA Kit (EFC1M) for mouse antibodies and Custom Cell-based ELISA Kit (EFC2R) for rabbit antibodies. All that is required is a primary antibody. If you prefer, we can develop the assay for you. Please email or call 1-510-782-9988 x 2 to request assay services

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Maniam, P., et al. (2015). Regulatory role of GSK3beta in the activation of NF-kB and modulation of cytokine levels in Burkholderia pseudomallei-infected PBMC isolated from streptozotocin-induced diabetic animals. Tropical biomedicine, 32(1), 36-48. Assay: NFKB phosphorylation in rat blood cell (PBMC).

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

NFKB Phosphorylation Assay Kit
$569.00

Out of stock

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

Related Products

You may also like…

Name SKU Price Buy
EnzyFluo™ Human/Mouse RB(T821/826) Phosphorylation ELISA Kit ERBT-100

$519.00

Diabetes & Obesity

EnzyFluo™ Human/Mouse RB(T821/826) Phosphorylation ELISA Kit

$519.00
- +
Add to cart
EnzyFluo™ Human/Mouse RPS6(S235/236) Phosphorylation ELISA ERPS6-100

$519.00

Diabetes & Obesity

EnzyFluo™ Human/Mouse RPS6(S235/236) Phosphorylation ELISA

$519.00
- +
Add to cart
EnzyFluo™ Human SHP2 (Y542) Phosphorylation ELISA Kit ESHP2-100

$519.00

Diabetes & Obesity

EnzyFluo™ Human SHP2 (Y542) Phosphorylation ELISA Kit

$519.00
- +
Add to cart
EnzyFluo™ Human/Mouse LKB1(S431) Phosphorylation ELISA Kit ELKB1-100

$519.00

Diabetes & Obesity

EnzyFluo™ Human/Mouse LKB1(S431) Phosphorylation ELISA Kit

$519.00
- +
Add to cart
EnzyFluo™ Human/Mouse GSK3B(S9) Phosphorylation ELISA Kit EGSK3B-100

$519.00

Diabetes & Obesity

EnzyFluo™ Human/Mouse GSK3B(S9) Phosphorylation ELISA Kit

$519.00
- +
Add to cart
EnzyFluo™ Human/Mouse GSK3A(S21) Phosphorylation ELISA Kit EGSK3A-100

$519.00

Diabetes & Obesity

EnzyFluo™ Human/Mouse GSK3A(S21) Phosphorylation ELISA Kit

$519.00
- +
Add to cart
EnzyFluo™ Human/Mouse GSK3A(S21) Phosphorylation ELISA Kit EGSK3A-100

$519.00

Diabetes & Obesity

EnzyFluo™ Human/Mouse GSK3A(S21) Phosphorylation ELISA Kit

$519.00
- +
Add to cart
EnzyFluo™ ERK Phosphorylation Assay Kit EERK-100

$579.00

Signal Transduction

EnzyFluo™ ERK Phosphorylation Assay Kit

$579.00
- +
Add to cart
EnzyFluo™ Custom Cell-Based ELISA Kit EFC1M-100

$379.00

Diabetes & Obesity

EnzyFluo™ Custom Cell-Based ELISA Kit

$379.00
- +
Add to cart
EnzyFluo™ Human/Mouse 4EBP1(S65) Phosphorylation ELISA Kit E4EBP-100

$519.00

Diabetes & Obesity

EnzyFluo™ Human/Mouse 4EBP1(S65) Phosphorylation ELISA Kit

$519.00
- +
Add to cart

Why BioAssay Systems

Quality and User-friendly • Expert Technical Support • Competitive Prices • Expansive Catalogue • Trusted Globally