Shopping Cart

EnzyFluo™ Human SHP2 (Y542) Phosphorylation ELISA Kit


  • For determination of SHP2(Y542) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA, etc.

Key Features

  • New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).
  • Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis is necessary.
  • Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.


  • Cell-Based ELISA (FL530/585 nm, 360/450 nm)


  • Cells


  • human


  • The assay takes 6.5 hrs, hands-on time 2.5 hrs


  • 100 tests

Shelf Life

  • 6 months

More Details

PROTEIN PHOSPHORYLATION plays a key role in regulating protein activity within cells. A large number of protein kinases and phosphatases are known to be involved in a variety of signal transduction pathways. A rapid assay capable of monitoring such signal transduction pathways, target protein phosphorylation status, and drug effects is highly desirable in life science research and drug discovery. Src Homology Region 2 Domain-containing Phosphatase-2 (SHP2, or SHPTP2, PTPN11, PTP2C) is a widely expressed protein-tyrosine phosphatase that plays regulatory roles in mitogenic activation, metabolic control, transcription regulation, and cell migration. BioAssay Systems’ SHP2 (Y542) Phosphorylation ELISA Kit provides a rapid and sensitive assay for measuring the phosphorylated protein pSHP2(Y542) in human cells and normalizes the level of phosphorylation to the total protein content in the same well. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study the underlying signal pathway and the effects of inhibitors, siRNA, or activators on the pathway. In this assay, cells grown in a 96-well plate are fixed and permeabilized. The phosphorylation of SHP2(Y542) is measured using a specific primary antibody followed by a HRP-conjugated secondary antibody with a fluorogenic substrate, and a fluorescent reagent that measures the total protein in the same well.

Haven’t Found the ELISA Kit for Your Target

We offer two DIY kits: Custom Cell-based ELISA Kit (EFC1M) for mouse antibodies and Custom Cell-based ELISA Kit (EFC2R) for rabbit antibodies. All that is required is a primary antibody. If you prefer, we can develop the assay for you. Please email or call 1-510-782-9988 x 2 to request assay service.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

No citations for this new product. Please check back later. You may click here to check if citations are available, but are not listed here yet.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

Human SHP2 (Y542) Phosphorylation ELISA Kit
- +

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

Why BioAssay Systems

Quality and User-friendly • Expert Technical Support • Competitive Prices • Expansive Catalogue • Trusted Globally