For determination of GSK3B(S9) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA, etc.
Key Features
New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).
Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis is necessary.
Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
Cell-Based ELISA (FL530/585 nm, 360/450 nm)
Samples
Cells
Species
human, rat and mouse
Procedure
Assay takes 6.5 hrs, hands-on time 2.5 hrs
Size
100 tests
Shelf Life
6 months
More Details
PROTEIN PHOSPHORYLATION plays a key role in regulating protein activity within cells. A large number of protein kinases and phosphatases are known to be involved in a variety of signal transduction pathways. A rapid assay capable of monitoring such signal transduction pathways, target protein phosphorylation status, and drug effects is highly desirable in life science research and drug discovery. Glycogen Synthase Kinase-3 beta (GSK3B, Gsk3b, 7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3. EC 2.7.11.1) plays an important role in energy metabolism, neuronal cell development, and body pattern formation. It is a novel therapeutic target for ischemic stroke. BioAssay Systems’ GSK3B(S9)Phosphorylation ELISA Kit provides a rapid and sensitive assay for measuring the phosphorylated protein pGSK3B(S9) in cultured cells and normalizes the level of phosphorylation to the total protein content in the same well. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study the underlying signal pathway and the effects of inhibitors, siRNA, or activators on the pathway. In this assay, cells grown in a 96-well plate are fixed and permeabilized. The phosphorylation of GSK3B(S9) is measured using a specific primary antibody followed by a HRP-conjugated secondary antibody with a fluorogenic substrate, and a fluorescent reagent that measures the total protein in the same well.
No frequently asked questions for this new product. Please check back later.
For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.
No citations for this new product. Please check back later. You may click here to check if citations are available, but are not listed here yet.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
Orders are shipped the same day if placed by 2pm PST Shipping: On Ice Carrier: Fedex Delivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C upon receipt