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EnzyFluo™ Human/Mouse RB(T821/826) Phosphorylation ELISA Kit


  • For determination of RB(T821/826) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc.

Key Features

  • New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).
  • Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary.
  • Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.


  • Cell-Based ELISA (FL530/585 nm, 360/450 nm)


  • Cells


  • human, rat and mouse


  • Assay takes 6.5 hrs, hands-on time 2.5 hrs


  • 100 tests

Shelf Life

  • 6 months

More Details

  • PROTEIN PHOSPHORYLATION plays key roles in regulating protein activity within cells. A large number of protein kinases and phosphatases are known to be involved in a variety of signal transduction pathways. A rapid assay capable of monitoring such signal transduction pathways, target protein phosphorylation status and drug effects is highly desirable in life science research and drug discovery.The RetinoBlastoma tumor suppressor protein (Rb, RB1, pRb, retinoblastoma 1, OSRC, PPP1R130, p105-Rb, pp110, retinoblastoma protein, RB transcriptional corepressor 1, p110-RB1) regulates cell differentiation, apoptosis and cell cycle control. BioAssay Systems’ RB(T821/826) Phosphorylation ELISA Kit provides a rapid and sensitive assay for measuring the phosphorylated protein pRB(T821/826) in cultured cells and normalizes the level of phosphorylation to the total protein content in the same well. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study the underlying signal pathway and effects of inhibitors, siRNA or activators on the pathway. In this assay, cells grown in a 96-well plate are fixed and permeabilized. The phosphorylation of RB(T821/826) is measured using a specific primary antibody followed by a HRP-conjugated secondary antibody with a fluorogenic substrate, and a fluorescent reagent that measures the total protein in the same well.

    Haven’t Found the ELISA Kit for Your Target

    We offer two DIY kits: Custom Cell-based ELISA Kit (EFC1M) for mouse antibody and Custom Cell-based ELISA Kit (EFC2R) for rabbit antibody. All that is required is a primary antibody. If you prefer, we can develop the assay for you. Please email or call 1-510-782-9988 x 2 to request assay service.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.


For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.

Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

Why BioAssay Systems?

Technical support provided by the very scientists that develop the assays.

Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Because we develop and manufacture the products, our prices are lower than competitors on the market

With over 200 different products, acquire all your assay kit needs in one order.

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