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QuantiChrom™ Glyoxalase I Assay Kit



Application

  • For quantitative determination of glyoxalase enzyme activity.

Key Features

  • Fast and sensitive. Detection limit of 4 U/L GLO-1 activity.
  • Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

  • OD240nm (Use a UV plate, e.g. 96-well UV plate, cat# P96UV)

Samples

  • Enzyme preparations or biological samples

Species

  • All

Procedure

  • 30 min

Size

  • 100 tests

Detection Limit

  • 4 U/L

Shelf Life

  • 12 months

More Details

GLYOXALASE I (GLO-1), a lactoylglutathione lyase also known as methylglyoxalase, aldoketomutase, ketone-aldehyde mutase, and (R)-S-lactoylglutathione methylglyoxal-lyase, is an enzyme that catalyzes the isomerization of hemithioacetal adducts which are formed in spontaneous reactions between glutathionyl groups and aldehydes. The primary physiological function of glyoxalase I is the detoxification of methylglyoxal, a reactive 2-oxoaldehyde that is cytostatic at low concentrations and cytotoxic at millimolar concentrations. Glyoxalase I is a target for the development of pharmaceuticals against bacteria, protozoans and human cancer. Simple, direct and automation-ready procedures for measuring GLO-1 activity in biological samples are highly desirable in research and drug discovery. BioAssay Systems Glyoxalase I assay kit provides a sensitive and convenient method for GLO-1 activity determination. The method involves monitoring the increase in the product of the GLO-1 reaction, S-lactoylglutathione, by measuring the change in absorbance at 240 nm.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

Glyoxalase I Assay Kit
$429.00
- +

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

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