QuantiChrom™ Alcohol Dehydrogenase Assay Kit
- For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.
- Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction.
- Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
- Biological (e.g. plasma, serum, urine, tissue, and culture media)
- 30 min
- 100 tests
- 0.4 U/L
- 6 months
More DetailsALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase that catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the breakdown of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. BioAssay System’s non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Capri, K. M., et al. (2020). Effects of time of day and constant light on the behavioral responses and ethanol metabolism to acute alcohol administration in male Black Swiss mice. Biological Rhythm Research 51(4): 566-585. Assay: Alcohol Dehydrogenase in mice.
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