EnzyChrom™ Glutathione Peroxidase Assay Kit

Protocol SDS

Application

• For quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity.

Key Features

• Sensitive and accurate. Use a 10 µL sample. Linear detection ranges from 40 to 800 U/L GPX activity.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD340nm

Samples

• Biological

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• 40 U/L

Shelf Life

• 6 months

More Details

Glutathione peroxidase (GPX, EC 1.11.1.9) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell. GPX catalyzes the following reaction with glutathione reductase (GR), Simple, direct, and high-throughput assays for GPX activity find wide applications. BioAssay Systems improved assay directly measures NADPH consumption in the enzyme-coupled reactions. The measured decrease in optical density at 340 nm is directly proportional to the enzyme activity in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Haubold, S et al. (2020). Effects of abomasal infusion of essential fatty acids and conjugated linoleic acid on performance and fatty acid, antioxidative, and inflammatory status in dairy cows. Journal of Dairy Science, 103(1): 972-991. Assay: Glutathione Peroxidase in bovine plasma.

de Maistre, S et al. (2020). Cecal metabolome fingerprint in a rat model of decompression sickness with neurological disorders. Scientific Reports, 10(1), 15996. Assay: Glutathione Peroxidase in rat plasma.

Pena-Bermudez, YA et al. (2020). Effects of feeding increasing levels of yerba mate on lamb meat quality and antioxidant activity. Animals: An Open Access Journal from MDPI, 10(9). Assay: Glutathione Peroxidase in lamb tissue.

Lopes-Pires, ME et al. (2020). Zinc regulates reactive oxygen species generation in platelets. Platelets: 1-10. Assay: Glutathione Peroxidase in human platelet suspensions.

Sanjay, S et al. (2021). Gallic acid attenuates isoniazid and rifampicin-induced liver injury by improving hepatic redox homeostasis through influence on Nrf2 and NF-kB signalling cascades in Wistar Rats. Journal of Pharmacy and Pharmacology, 73(4): 473-486. Assay: Glutathione Peroxidase in rat liver.

Hirahara, I et al. (2020). Hypermetabolism of glutathione, glutamate and ornithine via redox imbalance in methylglyoxal-induced peritoneal injury rats. Journal of Biochemistry, 167(2), 185-194. Assay: Glutathione Peroxidase in rat peritoneal effluent.

Vieira da Silva, I et al. (2021). Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets. PloS One, 16(1), e0245739. Assay: Glutathione Peroxidase in pig serum.

Humam, AM et al. (2020). Dietary supplementation of postbiotics mitigates adverse impacts of heat stress on antioxidant enzyme activity, total antioxidant, lipid peroxidation, physiological stress indicators, lipid profile and meat quality in broilers. Animals: An Open Access Journal from MDPI, 10(6). Assay: Glutathione Peroxidase in avian plasma.

Sharma, S et al. (2021). Heat-induced endoplasmic reticulum stress in soleus and gastrocnemius muscles and differential response to UPR pathway in rats. Cell Stress & Chaperones, 26(2): 323-339. Assay: Glutathione Peroxidase in rat muscle.

Magana-Cerino, JM et al. (2020). Consumption of nixtamal from a new variety of hybrid blue maize ameliorates liver oxidative stress and inflammation in a high-fat diet rat model. Journal of Functional Foods, 72, 104075. Assay: Glutathione Peroxidase in rat liver.

Humam, AM et al. (2021). Supplementation of postbiotic RI11 improves antioxidant enzyme activity, upregulated gut barrier genes, and reduced cytokine, acute phase protein, and heat shock protein 70 gene expression levels in heat-stressed broilers. Poultry Science, 100(3), 100908. Assay: Glutathione Peroxidase in avian plasma.

Kim, NY et al. (2020). Anti-inflammatory effects of ribes diacanthum pall mediated via regulation of nrf2/ho-1 and nf-kb signaling pathways in lps-stimulated raw 264. 7 macrophages and a tpa-induced dermatitis animal model. Antioxidants (Basel, Switzerland), 9(7). Assay: Glutathione Peroxidase in mouse cells.

Hsieh, P-S et al. (2021). Lactobacillus spp. Reduces ethanol-induced liver oxidative stress and inflammation in a mouse model of alcoholic steatohepatitis. Experimental and Therapeutic Medicine, 21(3), 188. Assay: Glutathione Peroxidase in mouse liver.

De Maistre, S., Vallee, N., Gaillard, S., Duchamp, C., & Blatteau, J. E. (2018). Stimulating fermentation by the prolonged acceleration of gut transit protects against decompression sickness. Scientific reports, 8(1), 10128. Assay: Glutahione peroxidase in rat plasma.

Medina-Leendertz, S., Mora, M., Vielma, J. R., Bravo, Y., Atencio-Bracho, L., Leal-Yepez, A. & Bonilla, E. (2018). Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese. Investigacion Clinica, 59(3), 230-241. Assay: Glutahione peroxidase in D. melanogaster tissues.

Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: Glutahione peroxidase in rat tissues.

Kook, S. H., Cheon, S. R., Kim, J. H., Choi, K. C., Kim, M. K., & Lee, J. C. (2017). Dietary hydroxycinnamates prevent oxidative damages to liver, spleen, and bone marrow cells in irradiation-exposed mice. Food science and biotechnology, 26(1), 279-285. Assay: Glutahione peroxidase in mice tissues.

Adeyemi, K. D., Ismail, M., Ebrahimi, M., Sabow, A. B., Shittu, R. M., Karim, R., & Sazili, A. Q. (2016). Fatty acids, lipid and protein oxidation, metmyoglobin reducing activity and sensory attributes of biceps femoris muscle in goats fed a canola and palm oil blend. South African Journal of Animal Science, 46(2), 139-151. Assay: Glutahione peroxidase in goat tissues.

Adeyemi, K. D., Sabow, A. B., Aghwan, Z. A., Ebrahimi, M., Samsudin, A. A., Alimon, A. R., & Sazili, A. Q. (2016). Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend. Journal of animal science and technology, 58(1), 6. Assay: Glutahione peroxidase in goat serum.

Adeyemi, K. D., Shittu, R. M., Sabow, A. B., Abubakar, A. A., Karim, R., Karsani, S. A., & Sazili, A. Q. (2016). Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. Journal of animal science and technology, 58(1), 23. Assay: Glutahione peroxidase in boer tissues.

Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Glutahione peroxidase in fish plasma.

Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Glutathione Peroxidase in fish plasma.

Ahmed, MA et al (2014). Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats. Life Sciences 110(2): 61-9. Assay: Glutahione peroxidase in rat brain tissue.

Chen, W et al (2014). Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats. PLoS One 9(2):e88076. Assay: Glutahione peroxidase in rat hippocampus tissue.

Effendy, NM and Shuid, AN (2014). Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis rat Model. Nutrients 6(8): 3288-302. Assay: Glutahione peroxidase in rat bone tissue.

Shabir, H et al (2014). Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol. Toxicological & Environmental Chemistry 96(2): 307-317. Assay: Glutahione peroxidase in rat.

Aghwan, ZA et al (2013). Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets. Asian-Australasian Journal of Animal Sciences (AJAS) 26.11: 1577-1582. Assay: Glutahione peroxidase in deer blood.

Aghwan, ZA et al (2013). Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets. Asian-Australasian Journal of Animal Sciences (AJAS) 26.11: 1577-1582. Assay: Glutahione Peroxdiase in Deer Blood.

Calamita G et al (2012). Biophysical assessment of aquaporin-9 as principal facilitative pathway in mouse liver import of glucogenetic glycerol. Biol Cell 104(6):342-51. Assay: Glutahione peroxidase in human blood.

Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Glutathione Peroxidase in human blood.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.