Protocol SDS

Application

• For quantitative determination of peroxidase enzyme activity.

Key Features

• Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD570nm, or FL530/585nm

Samples

• Biological

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• OD, FL: 4, 0.8 U/L

Shelf Life

• 12 months

More Details

PEROXIDASES (EC number 1.11.1.x) catalyzes the following oxidation-reduction reactions:
ROOR + electron donor (2 e-) + 2H⁺ → ROH + R’OH.
For many peroxidases, the optimal substrate is hydrogen peroxide (H₂O₂), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plants, fungi, and bacteria (cytochrome-c peroxidase-like enzymes such as horseradish peroxidase). Simple, direct, and automation-ready procedures for determining peroxidase activity find wide applications. BioAssay Systems peroxidase assay uses H₂O₂ and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (λex/em = 530/590nm) is a direct measure of the enzyme activity.duangwatch