Application

• For quantitative determination of peroxidase enzyme activity.

Key Features

• Fast and sensitive. Use as little as 10 µL samples. Linear detection range: colorimetric assays 2 to 50 U/L, fluorimetric assays 0.1 to 5 U/L peroxidase.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD570nm, or FL530/585nm

Samples

• Biological

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• OD, FL: 4, 0.8 U/L

Shelf Life

• 12 months

More Details

• PEROXIDASES (EC number 1.11.1.x) catalyze the following oxidation-reduction reactions:
  ROOR + electron donor (2 e-) + 2H⁺ → ROH + R’OH.
  For many peroxidases the optimal substrate is hydrogen peroxide (H₂O₂), but others are more active with organic hydroperoxides such as lipid peroxides. In the cell, peroxidases destroy toxic hydroxide radicals that are formed as byproducts during aerobic respiration. The peroxidases represent a large family of enzymes that are found in animals (e.g. myeloperoxidase-like enzymes), plant, fungi and bacteria (cytochrome-c peroxidase like enzymes such a horseradish peroxidase). Simple, direct and automation-ready procedures for determining peroxidase activity find wide applications. BioAssay Systems peroxidase assay uses H₂O₂ and an electron donor dye that forms a pink color during the peroxidase reaction. The optical density (570nm) or fluorescence intensity (λex/em = 530/590nm) is a direct measure of the enzyme activity.duangwatch

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to our General Questions

Wada, A., et al. (2021). Protective effect of luminal uric acid against indomethacin-induced enteropathy: Role of antioxidant effect and gut microbiota. Digestive Diseases and Sciences. Assay: Peroxidase in mouse ileal mucosa.

Singh, B., et al. (2020). Oxidative stress associated metabolic adaptations regulate radioresistance in human lung cancer cells. Journal of Photochemistry and Photobiology. B, Biology 213. Assay: Peroxidase in human lung cancer cells .

Jeon, G., et al. (2021). Melanin-decolorizing activity of antioxidant enzymes, glutathione peroxidase, thiol peroxidase, and catalase. Molecular Biotechnology 63(2): 150-155. Assay: Peroxidase in lysosomal fraction .

Sharma, S., et al. (2020). Heat-induced endoplasmic reticulum stress in soleus and gastrocnemius muscles and differential response to UPR pathway in rats. Cell Stress & Chaperones. Assay: Peroxidase in rat muscle .

Ismail, I. B., Al-Busadah, K. A., & El-Bahr, S. M. (2013). Oxidative stress biomarkers and biochemical profile in broilers chicken fed zinc bacitracin and ascorbic acid under hot climate. Am J Biochem Mol Biol, 3, 202-214. Assay: Peroxidase in broiler chicks liver cells.

Hokari, R., et al. (2011). Increased expression of lipocalin-type-prostaglandin D synthase in ulcerative colitis and exacerbating role in murine colitis. Am J Physiol Gastrointest Liver Physiol 300(3):G401-8. Assay: Peroxidase in mouse.

Jawed H et al (2010) N-(2-hydroxy phenyl) acetamide inhibits inflammation-related cytokines and ROS in adjuvant-induced arthritic (AIA) rats. Int Immunopharmacol 10(8):900-5 Assay: Peroxidase in rat serum.

Nguyen GN et al (2009) Drought-Induced Oxidative Conditions in Rice Anthers Leading to a Programmed Cell Death and Pollen Abortion. Journal of Agronomy and Crop Science 195(3):157-164. Assay: Peroxidase in plant rice anther.

Yu CL et al (2008) A novel caffeine dehydrogenase in Pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J Bacteriol 190(2):772-6 Assay: Peroxidase in bacterial cell.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.