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EnzyChrom™ GSH/GSSG Assay Kit


  • For quantitative determination of reduced/oxidized glutathione (GSH/GSSG) and evaluation of drug effects on their metabolism.

Key Features

  • Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.


  • OD412nm


  • Whole blood, plasma, serum, urine, tissue, and cell extracts


  • All


  • 20 min


  • 100 tests

Detection Limit

  • 10 nM

Shelf Life

  • 6 months

More Details

Glutathione, a tripeptide of glycine, glutamic acid, and cysteine, is one of the key antioxidants involved in protecting cells from damage by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. BioAssay Systems GSH/GSSG Assay Kit is designed to accurately measure total, reduced, and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol that first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.

I would like to measure cell lysate samples, but the kit doesn’t come with enough Scavenger.

We sell Scavenger separately, and the catalog number is EGTT-SCVG.

I need to deproteinate my sample, but don’t own any meta-phosphoric acid. Where can I get some?

We sell meta-phosphoric acid separately. The catalog number is MPA-2G.

Paul, S., et al. (2021). D4F prophylaxis enables redox and energy homeostasis while preventing inflammation during hypoxia exposure. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie 133: 111083. Assay: GSH/GSSG in rat lung tissue and plasma.

Shukla, M., et al. (2020). Moderate noise associated oxidative stress with concomitant memory impairment, neuro-inflammation and neurodegeneration. Brain, Behavior, & Immunity – Health 5: 100089. Assay: GSH/GSSG in rat brain hippocampus tissue.

Lee, J., et al. (2020). Epigenetic reprogramming of epithelial-mesenchymal transition promotes ferroptosis of head and neck cancer. Redox Biology 37: 101697. Assay: GSH/GSSG in mouse HNC cells and cancer tissue.

Gangwar, A., et al. (2020). Intermittent hypoxia modulates redox homeostasis, lipid metabolism associated inflammatory processes and redox post-translational modifications: Benefits at high altitude. Scientific Reports 10(1): 7899. Assay: GSH/GSSG in human plasma.

Bandara, A. Y., et al. (2019). Dynamics of host glutathione and glutathione related enzymes in Macrophomina phaseolina-sorghum bicolor interaction. BioRxiv 853986. Assay: GSH/GSSG in plant stalk tissue.

Hu, J., et al. (2020). Targeting Epstein-Barr virus oncoprotein LMP1-mediated high oxidative stress suppresses EBV lytic reactivation and sensitizes tumors to radiation therapy. Theranostics 10(26): 11921-11937. Assay: GSH/GSSG in human cells and serum.

Kim, H. J., et al. (2020). Neuroprotective activity of polyphenol-rich Ribes diacanthum pall against oxidative stress in glutamate-stimulated HT-22 cells and a scopolamine-induced amnesia animal model. Antioxidants (Basel, Switzerland) 9(9). Assay: GSH/GSSG in mouse cells.

Lin, T. A., Ke, B. J., Cheng, C. S., Wang, J. J., Wei, B. L., & Lee, C. L. (2019). Red Quinoa Bran Extracts Protects against Carbon Tetrachloride-Induced Liver Injury and Fibrosis in Mice via Activation of Antioxidative Enzyme Systems and Blocking TGF-beta1 Pathway. Nutrients, 11(2), 395. Assay: GSH/GSSG in mice tissues.

Kim, K. H., Kim, E. Y., Lee, S. Y., Ko, J. J., & Lee, K. A. (2018). Oocyte Cytoplasmic Gas6 and Heparan Sulfate (HS) are Required to Establish the Open Chromatin State in Nuclei During Remodeling and Reprogramming. Cellular Physiology and Biochemistry, 45(1), 37-53. Assay: GSH/GSSG in mice cells.

Li, X. X., Wang, Z. J., Zheng, Y., Guan, Y. F., Yang, P. B., Chen, X. & Chien, K. Y. (2018). Nuclear receptor Nur77 facilitates melanoma cell survival under metabolic stress by protecting fatty acid oxidation. Molecular cell, 69(3), 480-492. Assay: GSH/GSSG in mice cells.

Medina-Leendertz, S., Mora, M., Vielma, J. R., Bravo, Y., Atencio-Bracho, L., Leal-Yepez, A. & Bonilla, E. (2018). Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese. Investigacion Clinica, 59(3), 230-241. Assay: GSH/GSSG in D. melanogaster tissues.

Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2018). STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox biology, 14, 423-438. Assay: GSH/GSSG in rat tissues and plasma.

Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: GSH/GSSG in human tissues.

Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2017). Deciphering Molecular Cascades In A Novel Acclimatization Strategy For Rapid Ascent To High Altitude. bioRxiv, 145342. Assay: GSH/GSSG in rat tissues and plasma.

Khatri, R., Krishnan, S., Roy, S., Chattopadhyay, S., Kumar, V., & Mukhopadhyay, A. (2016). Reactive oxygen species limit the ability of bone marrow stromal cells to support hematopoietic reconstitution in aging mice. Stem cells and development 25(12):948-58. Assay: GSH/GSSG in mice stromal cells.

Lee, H. N., et al. (2015). Heme Oxygenase-1 Determines the Differential Response of Breast Cancer and Normal Cells to Piperlongumine. Molecules and cells, 38(4), 327. Assay: GSH/GSSG in piperlongumine cell.

Chatterjee, N., Eom, H. J., & Choi, J. (2014). A systems toxicology approach to the surface functionality control of graphene-cell interactions. Biomaterials, 35(4), 1109-1127. Assay: GSH/GSSG in human HepG2 cells.

Di Maro, G., et al. (2014). Anterior gradient protein 2 promotes survival, migration and invasion of papillary thyroid carcinoma cells. Molecular cancer, 13(1), 1. Assay: GSH/GSSG in human tissues.

Muller, S., et al. (2014). Age-dependent effects of UCP2 deficiency on experimental acute pancreatitis in mice. PloS one, 9(4), e94494. Assay: GSH/GSSG in mice serum.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

Glutathione GSH/GSSG Assay Kit
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For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

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