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EnzyChrom™ GSH/GSSG Assay Kit



Application

  • For quantitative determination of reduced/oxidized glutathione (GSH/GSSG) and evaluation of drug effects on their metabolism.

Key Features

  • Sensitive and accurate. Linear detection range 0.01-3 µM GSH equivalents with a detection limit of 10 nM GSH equivalents.

Method

  • OD412nm

Samples

  • Whole blood, plasma, serum, urine, tissue, and cell extracts

Species

  • All

Procedure

  • 20 min

Size

  • 100 tests

Detection Limit

  • 10 nM

Shelf Life

  • 6 months

More Details

Glutathione, a tripeptide of glycine, glutamic acid, and cysteine, is one of the key antioxidants involved in protecting cells from damage by reactive oxygen species. Glutathione (GSH) reduces disulfide bonds in cytoplasmic proteins to cysteines, in which it is converted to its oxidized form GSSG. BioAssay Systems GSH/GSSG Assay Kit is designed to accurately measure total, reduced, and oxidized glutathione in biological samples using an enzymatic method that utilizes Ellman’s Reagent (DTNB) and glutathione reductase (GR). DTNB reacts with reduced glutathione to form a yellow product. The rate of change in the optical density, measured at 412 nm, is directly proportional to glutathione concentration in the sample. This kit can also be used to measure oxidized (GSSG) by using a specific protocol that first scavenges all GSH with 1-methyl-2-vinylpyridinium triflate.

Weight 15 lbs
Dimensions 15 × 13 × 9 in

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