EnzyChrom™ Nitric Oxide Synthase Inhibitor Screening Kit

Protocol SDS

Application

• For evaluation and high-throughput screen (HTS) of nitric oxide synthase (NOS) modulators.

Key Features

• High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system.
• Rapid and reliable. Can be completed in less than 3 hours if the assay is performed at 37°C.

Method

• OD540nm

Samples

• Nitric Oxide Synthase

Species

• All

Procedure

• 2-3 hours

Size

• 100 tests

Shelf Life

• 6 months

More Details

Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO is the oxidation product of arginine by nitric oxide synthase (NOS) and is involved in host defense development, activation of regulatory proteins, and direct covalent interaction with functional biomolecules. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system. Simple, direct, and non-radioactive procedures for measuring NOS are becoming popular in research and drug discovery. BioAssay Systems EnzyChrom™ Nitric Oxide Synthase Inhibitor Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following the reduction of nitrate to nitrite using an improved Griess method.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.