EnzyChrom™ Xanthine Oxidase Assay Kit

Protocol SDS

Application

• For quantitative determination of xanthine oxidase enzyme activity and evaluation of its modulators.

Key Features

• Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 20 minute incubation: 0.03 to 25 U/L xanthine oxidase for colorimetric assays and 0.01 to 2.5 U/L for fluorimetric assays.
• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.

Method

• OD570nm, FL530/585nm

Samples

• Cell lysate, serum, and other biological samples

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• FL 0.01 U/L; OD 0.03 U/L

Shelf Life

• 6 months

More Details

Xanthine Oxidase catalyzes the oxidation of xanthine to uric acid. In addition, xanthine oxidase can catalyze the oxidation of hypoxanthine to xanthine, act on certain purines and aldehydes, and in certain cases produce the superoxide ion. Clinically, xanthine oxidase activity in the blood can act as a marker for influenza, liver damage, and possibly cardiovascular health. Simple, direct and high-throughput assays for measuring xanthine oxidase activity find wide applications in research and drug discovery. BioAssay Systems xanthine oxidase assay kit uses a single Working Reagent that combines the xanthine oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to xanthine oxidase activity in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

El-Shennawy, L et al. (2020). Dose-dependent reproductive toxicity of sodium benzoate in male rats: Inflammation, oxidative stress and apoptosis. Reproductive Toxicology, 98: 92-98. Assay: Xanthine Oxidase in rat testes.

Sharma, S et al. (2020). Ameliorative effect of a standardized polyherbal combination in methotrexate-induced nephrotoxicity in the rat. Pharmaceutical Biology, 58(1): 184-199. Assay: Xanthine Oxidase in plant extract inhition.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.