EnzyChrom™ Xanthine Assay Kit

Protocol SDS

Application

• For quantitative determination of xanthine and drug effects on its metabolism.

Key Features

• Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 30-minute incubation: 0.01 to 2.5 mM xanthine for colorimetric assays and 3 to 250 µM for fluorimetric assays.
• Simple and convenient. The procedure involves addition of a single working reagent and incubation for 30 min at room temperature.
• Fast and high-throughput. Assays using 96-well plates and a liquid handling system could allow simultaneous processing of tens of thousands of samples per day.

Method

• OD570nm, FL530/585nm

Samples

• Cell lysate, serum, and other biological samples

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• FL 3 µM; OD 10 µM

Shelf Life

• 6 months

More Details

Xanthine is a purine base that can be found in most animal tissues and fluids. It is a product in the purine degradation pathway, produced by guanine deaminase from guanine, and by xanthine oxidoreductase from hypoxanthine. Xanthine is degraded to uric acid by xanthine oxidase. Clinically, xanthine and its derivatives act on sleep-inducing adenosine receptors as antagonists. Simple, direct, and high-throughput assays for measuring xanthine find wide applications in research and drug discovery. BioAssay Systems xanthine assay kit uses a single Working Reagent that combines the xanthine oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the xanthine concentration in the sample.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.