QuantiChrom™ Total Protein Assay Kit

Protocol SDS

Application

• For quantitative determination of total protein.

Key Features

• Fast and sensitive. The assay takes 10 min. Linear detection range of 5 – 200 mg/dL protein.
• Robust and Reliable. Substances in urine have minimal interference (< 2%).
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD600nm (Pyrogallol Red)

Samples

• Urine, cerebrospinal fluid, etc

Species

• All

Procedure

• 10 min

Size

• 100 tests

Detection Limit

• 5 mg/dL

Shelf Life

• 6 months

More Details

Total protein concentration can be determined by several methods. However, protein dye-binding procedures are simple, reliable, rapid, and readily automated and are becoming increasingly popular in assessing renal and blood-brain barrier functions. BioAssay Systems Total Protein Assay Kit is based on an improved pyrogallol red-molybdate protein dye-binding assay. The color intensity at 600nm is directly proportional to the total protein concentration in the sample. This single reagent can be used for the quantitative determination of total protein in urine (proteinuria) and cerebrospinal fluid (blood-brain barrier).

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Yasuda, N (2007). Effects of Long-term Strenuous Exercise onOxidative DNA Damage and Proteinuria in humans. GraduateStudent Theses, Dissertations, & Professional Papers. 362. Assay: DNA in rat primary glial culture.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.