EnzyChrom™ Sorbitol Assay Kit

Protocol SDS

Application

• For quantitative determination of D-sorbitol and evaluation of drug effects on sorbitol metabolism.

Key Features

• Fast and sensitive. Linear detection range (20 µL sample): 5 to 1000 µM D-sorbitol.
• Convenient and high-throughput. Room temperature assay. No 37°C incubator is required. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological (e.g. blood), food, beverage, and agriculture samples

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 5 µM

Shelf Life

• 6 months

More Details

SORBITOL (glucitol) is a sugar alcohol that is metabolized slowly in the human body. Sorbitol can be obtained from glucose by reducing the aldehyde group to a hydroxyl group. Accumulation of excessive sorbitol in erythrocytes, retinal cells, and Schwann cells has been associated with retinopathy, cataracts, peripheral neuropathy, and diabetes. Sorbitol is made solely from corn syrup, and found in fruits such as apples, pears, peaches, and prunes. It is widely used as a sugar substitute and as a laxative. It is also utilized in specialty culture media and in healthcare, food, and cosmetic products. Sorbitol is measured in biological samples to monitor metabolic pathways and the progression of diabetes. BioAssay Systems sorbitol assay involves an end-point enzyme coupled MTT/NAD reaction that forms a colored product with an absorption maximum of 565 nm. The increase in absorbance at 565 nm is directly proportional to the sorbitol concentration.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Kikuchi, K et al (2020). Diabetic cataract in spontaneously diabetic torii fatty rats. Journal of Diabetes Research, 2020, 3058547. Assay: Sorbitol in rat lens.

Sedlak, L et al (2018). Effect of Resveratrol, a Dietary-Derived Polyphenol, on the Oxidative Stress and Polyol Pathway in the Lens of rats with Streptozotocin-Induced Diabetes. Nutrients, 10(10), 1423. Assay: Sorbitol in rat cell lysate, lens.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.