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EnzyChrom™ Lactose Assay Kit


  • For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.

Key Features

  • Sensitive. Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays.
  • Convenient and high-throughput. Robust and homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.


  • OD570nm, or FL530/585nm


  • Biological (milk), food and agriculture, etc


  • All


  • 30 min


  • 100 tests

Detection Limit

  • 6 µM

Shelf Life

  • 6 months

More Details

Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct, and high-throughput assays for lactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.

Weight 15 lbs
Dimensions 15 × 13 × 9 in


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