Protocol SDS

Application

• For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.

Key Features

• Sensitive. Use as little as 20 µL samples. Linear detection range in 96-well plate: 17 to 2000 µM lactose for colorimetric assays and 6 to 100 µM for fluorimetric assays.
• Convenient and high-throughput. Robust and homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD570nm, or FL530/585nm

Samples

• Biological (milk), food and agriculture, etc

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 6 µM

Shelf Life

• 6 months

More Details

Lactose (C₁₂H₂₂O₁₁), also called milk sugar, is a disaccharide that consists of β-D-galactose and α/β-D-glucose through a β1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct, and high-throughput assays for lactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.