EnzyFluo™ Isocitrate Assay Kit

Protocol SDS

Application

• For quantitative fluorimetric determination of isocitrate and evaluation of drug effects on its metabolism.

Key Features

• Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL530/585 nm

Samples

• Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

Species

• All

Procedure

• 10 min

Size

• 100 tests

Detection Limit

• 0.6 µM

Shelf Life

• 6 months

More Details

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices. BioAssay Systems’ isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.