QuantiChrom™ Protein Assay Kit

Protocol SDS

Application

• For quantitative determination of total protein.

Key Features

• Sensitive and accurate. Use 10 mL samples. Detection range 0.06 – 1.0 mg /mL protein in 96-well plate assay.
• Simple and high-throughput. The “mix-and-read” procedure involves addition of a single working reagent and reading the optical density. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.
• Low interference. Glucose, Tris, vitamins, and amino acids, DNA, RNA, salts, EDTA (< 12 mM), phenol (< 50 mM), urea (< 0.6 M), Triton (<0.1%) and SDS (< 0.1% SDS) do not interfere in the assay.
• Versatility: assays can be executed in 96-well plate or cuvet.

Method

• OD595nm (Bradford)

Samples

• Biological, food, agriculture etc

Species

• All

Procedure

• 2 min

Size

• 500 tests

Detection Limit

• 60 µg/mL

Shelf Life

• 12 months

More Details

The protein is known as the “building blocks of life” and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quantitation is a very common practice for life scientists. Simple, direct and automation-ready procedures for measuring protein concentration are very desirable. BioAssay Systems QuantiChrom™ protein assay kit is based on an improved Coomassie Blue G method. The dye forms a blue complex specifically with protein, and the intensity of color, measured at 595nm, is directly proportional to the protein concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits increased sensitivity towards peptides.

Your protein assay kit detection range is 60-1000 µg/ml, is it possible to improve the kit sensitivity?

Simply adding 50 μL sample to 200 μL reagent increases the sensitivity to 3-200 mg/mL protein.

Cucchiarini M et al (2011). Metabolic activities and chondrogenic differentiation of human mesenchymal stem cells following recombinant adeno-associated virus-mediated gene transfer and overexpression of fibroblast growth factor 2. Tissue Eng Part A. 17(15-16):1921-33. Assay: Protein in bacteria p450 enzymes.

Sharifuzzaman SM et al (2011). Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum. Fish Shellfish Immunol. 30(1):347-53. Assay: Protein in human urine.

Sharifuzzaman SM, et al (2011). Subcellular components of probiotics Kocuria SM1 and Rhodococcus SM2 induce protective immunity in rainbow trout (Oncorhynchus mykiss, Walbaum) against Vibrio anguillarum. Fish Shellfish Immunol. 30(1):347-53. Assay: Protein in bacteria cells.

Waheed MM, et al (2011). Some Biochemical Characteristics and Preservation of Epididymal Camel Spermatozoa (Camelus dromedarius). Theriogenology 76(6):1126-33. Assay: Protein in animal testicles.

Olsen AS et al (2010). Limb regeneration is impaired in an adult zebrafish model of diabetes mellitus. Wound Repair Regen. 18(5):532-42. Assay: Protein in rat tissue.

Sharifuzzaman SM, Austin B (2010). Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). J Appl Microbiol. 108(6):2162-70. Assay: Protein in fish serum.

Sharifuzzaman SM, Austin B (2010). Kocuria SM1 controls vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). J Appl Microbiol. 108(6):2162-70. Assay: Protein in rat tissue.

Thanissery R et al (2010). Evaluation of the efficacy of yeast extract in reducing intestinal Clostridium perfringens levels in broiler chickens. Poult Sci. 89(11):2380-8. Assay: Protein in rat brown fat tissue.

Aldag C et al (2009). Probing the role of the proximal heme ligand in cytochrome P450cam by recombinant incorporation of selenocysteine. PNAS 106(14):5481-6. Assay: Protein in Bacteria cells.

Sharifuzzaman SM, Austin B (2009). Influence of probiotic feeding duration on disease resistance and immune parameters in rainbow trout. Fish Shellfish Immunol. 27(3):440-5. Assay: Protein in fish.

Stefanidis A, et al (2009). The role of thermogenesis in antipsychotic drug-induced weight gain. Obesity (Silver Spring) 17(1):16-24. Assay: Protein in rat brown fat tissue.

Verty AN, et al (2009). The effects of rimonabant on brown adipose tissue in rat: implications for energy expenditure. Obesity (Silver Spring) 17(2):254-61. Assay: Protein in rat tissue.

Lai, M. et al (2007). 2007 AAPS Annual Meeting & Exposition. Development of an In-Vitro Screening Method to Determine Enzymatic Release rates from a Homologous Series of Novel SN-38 Conjugates. Sonus Pharmaceuticals. Assay: Protein in rat tissue.

Yasuda, N (2007). Effects of Long-term Strenuous Exercise onOxidative DNA Damage and Proteinuria in humans. GraduateStudent Theses, Dissertations, & Professional Papers. 362. Assay: DNA in rat primary glial culture.

Yasuda, N. (2007). Effects Of Long-Term Strenuous Exercise On Oxidative Dna Damage And Proteinuria In humans. Ph.D. Thesis. Assay: Protein in human urine.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.