EnzyChrom™ Phosphoglucomutase Assay Kit

Protocol SDS

Application

• For quantitative determination of phosphoglucomutase enzyme activity in biological samples.

Key Features

• Safe and sensitive. Non-radioactive assay. Linear detection range in 96-well plate: 0.5 to 20 U/L activity.
• Fast and convenient. The procedure involves addition of a single working reagent and incubation for 20 min. Room temperature assay. No 37°C incubator is needed.
• High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.

Method

• OD460nm

Samples

• Serum, plasma, cell lysate, and other biological samples

Species

• All

Procedures

• 20 min

Size

• 100 tests in 96-well plate

Detection Limit

• 0.5 U/L

Shelf Life

• 6 months

More Details

PHOSPHOGLUCOMUTASE (E.C. 5.4.2.2) is an enzyme which catalyzes the interconversion of glucose-1-phosphate (G1P) and glucose-6-phosate (G6P). Phosphoglucomutase (PGM) plays a crucial role in glycogen synthesis and degradation. PGM 1 deficiency can cause a rare congenital disorder of glycosylation. A decrease in PGM activity has also been linked with oxidative stress. In BioAssay Systems’ EPGM-100 assay, PGM converts G1P to G6P, which is then oxidized by glucose-6-phosphate dehydrogenase. The formed NADPH is coupled to a formazan chromogen. The increase in absorbance at 460 nm is directly proportional to phosphoglucomutase activity.

Does this kit come with phosphoglucomutase?

No, this kit does not come with a phosphoglucomutase positive control.

What was the source of the enzyme used to develop this kit?

Phosphoglucomutase from rabbit skeletal muscle was used when developing this kit.

What cell count should I use when preparing cell lysate?

Our general guideline is to suspend about two million (2 x 10^6) harvested cells in 400 μL PBS on ice, then run a pilot test of the sample at different dilutions to determine the optimal one. When testing this kit with PANC-1 cell lysate, we used 650,000 cells/mL. However, the optimal number may vary depending on your cell line and samples.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.