This kit is shipped on ice. Upon receiving, please keep the kit in the freezer (-20°C).
The kit has been tested on PANC-1, HL-60, F0-B16, and NIH-3T3 cell lines. It has been tested with DMEM and RPMI media. It has also been tested with 2-deoxy-D-glucose inhibition of glycolysis.
For adherent cells, plate cells at desired cell density in media of choice. After allowing cells enough time to adhere to plate, remove media and replace with low percentage FBS media. Apply any treatments being tested at this time. Allow cells to propagate to desired confluency. Remove media for assay.
For suspension cells, seed cells in low percentage FBS media and apply any treatments being tested. Allow cells to grow to desired cell density. Remove media for assay.
The kit has been tested on and works with suspension cells. Although it is not necessary to spin them down, we have found spinning them down prior to assay leads to more consistent results.
The assay does work with 10% FBS media; however, it loses a significant amount of sensitivity due to high background. If one chooses to run the assay with 10% FBS media, pilot experiments may be needed to determine optimal conditions. We strongly recommend simply using a low percentage FBS (FBS <1%) or serum free media.
The assay standards reach ODs greater than 1.0. If your reader is not accurate to OD 2.0, we suggest making a modified set of standards at concentrations 0, 1.5, 3, and 5mM. Samples may need to be diluted in media so they fall within this range and be corrected for dilution factor in calculations.
Yes, it is highly recommended.
Yes, unused reagents can be stored according to the assay protocol. Excessive freeze/thaw cycles of reagents should be avoided.
Umezu, R., et al. (2020). Macrophage (Drp1) dynamin-related protein 1 accelerates intimal thickening after vascular injury. Arteriosclerosis, Thrombosis, and Vascular Biology, 40(7), e214-e226. Assay: Glycolysis in mouse cell media.
Moschovaki Filippidou, F., et al (2020). Glucagon-like peptide-1 receptor agonism improves nephrotoxic serum nephritis by inhibiting t-cell proliferation. The American Journal of Pathology, 190(2), 400-411 Assay: Glycolysis in mouse T cells.
Bernhart, E., et al (2010). Lysophosphatidic acid receptor activation affects the C13NJ microglia cell line proteome leading to alterations in glycolysis, motility, and cytoskeletal architecture. Proteomics, 10(1), 141-158. Assay: Glycolysis in cell media.
Kusuoka, Osamu, et al (2018). Intermittent calorie restriction enhances epithelial-mesenchymal transition through the alteration of energy metabolism in a mouse tumor model. International journal of oncology 52.2: 413-423. Assay: Glycolysis in mice cells.
Helsel, Aileen R., Melissa J. Oatley, and Jon M. Oatley(2017). Glycolysis-optimized conditions enhance maintenance of regenerative integrity in mouse spermatogonial stem cells during long-term culture. Stem cell reports 8.5: 1430-1441. Assay: Glycolysis in mice cell culture.
To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.
Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
Technical support provided by the very scientists that develop the assays.
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Because we develop and manufacture the products, our prices are lower than competitors on the market
With over 200 different products, acquire all your assay kit needs in one order.
Products used by clients worldwide with distributors in over 60 countries.
BioAssay Systems is committed to producing innovative, high-quality and cost-effective products and to providing expert technical service to our valued customers.