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EnzyChrom™ Hexokinase Assay Kit


  • Hexokinase activity determination in biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue and culture media.)

Key Features

  • Fast and sensitive. Linear detection range (20 μL sample): 0.22 to 100 U/L for 15 min reaction.
  • Convenient. Homogeneous “mix-incubate-measure” type assay.
  • High-throughput. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.


  • OD565nm


  • Biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue, and culture media.)


  • All


  • 15 min


  • 100 tests in 96-well plate

Detection Limit

  • 0.22 U/L

Shelf Life

  • 6 months

More Details

HEXOKINASE (E.C. is a mitochondrial and cytosolic enzyme and the first committed step in the glycolysis pathway. Hexose sugars, mainly glucose, are phosphorylated using ATP to form glucose-6-phosphate. Hexokinases are implicated in a myriad of human diseases, including cancer and diabetes. BioAssay Systems’ non-radioactive, colorimetric Hexokinase assay involves a stepwise reaction based on the reduction of the tetrazolium salt MTT in an NADPH-coupled enzymatic reaction to a colored product which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Weight 1 lbs
Dimensions 6 × 4 × 3 in


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