EnzyChrom™ Malate Assay Kit

Protocol SDS

Application

• For quantitative determination of L-malate (L-malic acid) and drug effects on malate metabolism.

Key Features

• Fast and sensitive. Use of 20 µL sample. Linear detection range 0.02 to 2 mM L-malate in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent and reading the optical density at a time of 15 minutes. Room temperature assay. No 37°C heater is needed.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• OD565nm

Samples

• Malate in food, juice, beverage, and other agricultural products

Species

• All

Procedure

• 15 min

Size

• 100 tests

Detection Limit

• 0.02 mM

Shelf Life

• 6 months

More Details

L-malate, or L-Malic acid, is a dicarboxylic acid that is made by all living organisms and plays an important role in the Calvin and Krebs Cycle. It is a source of CO₂ for the Calvin cycle in plants and is also an intermediate that forms from fumarate in the Krebs Cycle. Malate is frequently used in food and beverage industries as an additive in products such as wine, beer, candies, etc. BioAssay Systems L-malate assay kit is based on malate dehydrogenase catalyzed oxidation of malate in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the product color, measured at 565 nm is proportional to the malate concentration in the sample.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.