EnzyChrom™ Malate Dehydrogenase Assay Kit

Protocol SDS

Application

• For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.

Key Features

• Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 65 U/L for 20 min reaction at 37°C.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological samples (e.g. plasma, serum, erythrocytes, tissue, and culture media)

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 0.5 U/L

Shelf Life

• 6 months

More Details

Malate dehydrogenase (MDH) (EC 1.1.1.37) is an enzyme that reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 is found in the cytoplasm and plays a key part in the malate-aspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme that participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer’s disease. BioAssay Systems’ non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Venkat, S. (2020). Implications of lysine acetylation on the enzymes of the tca cycle. Theses and Dissertations. Assay: Malate Dehydrogenase in purified malate dehydrogenase.

Venkat, S. et al (2017). Genetically encoding thioacetyl-lysine as a non-deacetylatable analog of lysine acetylation in Escherichia coli. FEBS open bio, 7(11), 1805-1814. Assay: Malate dehydrogenase in E.coli cell lysate.

Gan, Q, Lehman, BP, Bobik, TA and Fan, C ( 2016) Expanding the genetic code of Salmonella with non-canonical amino acids. Sci Rep 6, 39920. Assay: Malate dehydrogenase in Salmonella cell lysate.

Fan, C et al (2015). rationally evolving tRNAPyl for efficient incorporation of noncanonical amino acids. Nucl. Acids Res. 43(22):e156. Assay: Malate dehydrogenase in E.coli cells.

Trivedi, AK et al (2015). Adaptation of oxidative phosphorylation to photoperiod-induced seasonal metabolic states in migratory songbirds. Comp. Biochem. Physiol. A 184: 34-40. Assay: Malate dehydrogenase in avian plasma.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.