Shopping Cart

EnzyChrom™ L-Alanine Assay Kit



Application

  • For quantitative determination of L-Alanine and evaluation of drug effects on alanine metabolism.

Key Features

  • Sensitive and accurate. Linear detection range in 96-well plate: 1 to 200 µM alanine for colorimetric assays and 0.4 to 20 µM for fluorimetric assays.

Method

  • OD570nm, or FL530/585nm

Samples

  • Plasma, serum, urine, tissue and culture media

Species

  • All

Procedure

  • 60 min

Size

  • 100 tests

Detection Limit

  • 0.4 µM

Shelf Life

  • 6 months

More Details

ALANINE , a nonessential amino acid, is utilized in the glucose-alanine cycle between tissues and the liver. In tissues that metabolize amino acids, amino groups are collected as glutamate by transamination. The amine group is then transferred by alanine transaminase (ALT) from glutamate to pyruvate to form alanine and a-ketoglutarate. The alanine generated is transported to the liver where a reverse ALT reaction occurs and pyruvate is regenerated. Pyruvate is converted through gluconeogenesis to glucose which can then be recirculated to the tissues. Alanine concentration may have some correlation with high blood pressure, energy intake, cholesterol levels and body mass index. BioAssay Systems’ Alanine Assay Kit provides a simple, direct and automation-ready procedure for measuring alanine concentration. Alanine is converted into pyruvate which can then be directly measured. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to the alanine concentration in the sample.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Jubouri, M. (2020). The effects of alanine on glucose metabolism in rainbow trout: Integration of glucose fluxes and molecular evidence [Thesis, Universite d’Ottawa / University of Ottawa]. Assay: L-alanine in fish plasma.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.

L-Alanine Assay Kit
$429.00
- +

For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.

Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

Related Products

You may also like…

NameSKUPriceBuy
EnzyChrom™ Alanine Transaminase Assay Kit EALT-100

$369.00

Blood & Urine Chemistry

EnzyChrom™ Alanine Transaminase Assay Kit

$369.00
- +
Add to cart
EnzyChrom™ Aspartate Assay Kit EASP-100

$429.00

Blood & Urine Chemistry

EnzyChrom™ Aspartate Assay Kit

$429.00
- +
Add to cart
EnzyChrom™ Choline Assay Kit ECHO-100

$449.00

Agriculture & Environment

EnzyChrom™ Choline Assay Kit

$449.00
- +
Add to cart
EnzyChrom™ Xanthine Assay Kit EXAN-100

$369.00

Agriculture & Environment

EnzyChrom™ Xanthine Assay Kit

$369.00
- +
Add to cart
EnzyChrom™ Oxalate Assay Kit EOXA-100

$589.00

Agriculture & Environment

EnzyChrom™ Oxalate Assay Kit

$589.00
- +
Add to cart
EnzyChrom™ Fumarate Assay Kit EFUM-100

$429.00

Agriculture & Environment

EnzyChrom™ Fumarate Assay Kit

$429.00
- +
Add to cart
EnzyChrom™ Citrate Assay Kit ECIT-100

$449.00

Agriculture & Environment

EnzyChrom™ Citrate Assay Kit

$449.00
- +
Add to cart
EnzyChrom™ L-Lactate Assay Kit ECLC-100

$459.00

Agriculture & Environment

EnzyChrom™ L-Lactate Assay Kit

$459.00
- +
Add to cart

Why BioAssay Systems

Quality and User-friendly • Expert Technical Support • Competitive Prices • Expansive Catalogue • Trusted Globally