EnzyChrom™ Fumarate Assay Kit

Protocol SDS

Application

• For quantitative determination of fumarate and drug effects on its metabolism.

Key Features

• Fast and sensitive. Use of 20 µL sample. Linear detection range 0.005 to 2 mM fumarate in a 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent and reading the optical density after 30 minutes. Room temperature assay. No 37°C heater is needed.
• High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• OD565nm

Samples

• Food, beverage and other biological samples (e.g. cell lysate, tissue homogenate, serum)

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 5 µM

Shelf Life

• 6 months

More Details

FUMARATE, or Fumaric Acid, is one of the key components in the TCA cycle and is used by cells to form ATP. Human skin when exposed to sunlight will naturally produce fumaric acid. Fumarate is used as an additive by the food and beverage industries. Fumaric acid esters are also used to treat psoriasis. Increased urinary fumarate may be due to impaired Krebs cycle function, a defect in the enzyme fumarase, or mitochondrial function. BioAssay Systems fumarate assay kit is based on fumarase-catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm is proportional to the fumarate concentration in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Kurabayashi, A et al. (2020). Murine remote ischemic preconditioning suppresses diabetic ketoacidosis by enhancing glycolysis and entry into tricarboxylic acid cycle in the liver. Life Sciences, 253, 117748. Assay: Fumarate in mouse liver.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.