EnzyChrom™ Aspartate Assay Kit

Protocol SDS

Application

• For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.

Key Features

• Sensitive and accurate. Linear detection range: 2 to 400 µM aspartate for colourimetric assays and 1 to 50 µM for fluorimetric assays.

Method

• OD570nm, or FL530/585nm

Samples

• Plasma, serum, tissue, culture media etc

Species

• All

Procedure

• 60 min

Size

• 100 tests

Detection Limit

• OD, FL: 2, 1 µM

Shelf Life

• 6 months

More Details

ASPARTATE, a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis. BioAssay Systems Aspartate Assay Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the aspartate concentration in the sample.

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For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.