EnzyChrom™ Histamine Assay Kit

Protocol SDS

Application

• For quantitative determination of histamine in food, beverage, and agricultural products.

Key Features

• Fast and sensitive. Use of 20 µL sample. Linear detection range 0.0025 – 1 mM (0.3 – 111 ppm) histamine in 96-well plate assay.
• Convenient. The procedure involves adding a single working reagent and reading the absorbance after 30 minutes. Room temperature assay. No 37°C heater is needed.
• High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

• OD565nm

Samples

• Food, beverage, and agricultural products

Species

• All

Procedure

• 30 min

Size

• 100 tests

Detection Limit

• 2.5 µM

Shelf Life

• 6 months

More Details

HISTAMINE (C₅H₉N₃) is a biogenic amine naturally present in many foods and body cells in amounts without toxicological significance. It is also a contaminant that is mostly found in the Scombridae family fishes such as tuna and mackerel or other seafood products when improperly handled or stored. The consumption of foods containing high levels of histamine may lead to an allergy-like food poisoning known as scombroid poisoning.BioAssay Systems’ histamine assay kit is based on histamine dehydrogenase catalyzed oxidation of histamine in which the formed electron mediator reduces a formazan (MTT) reagent. The intensity of product color, measured at 565 nm is directly proportional to histamine concentration in the sample.

Does the assay kit work in particular species?

Our assays work in all species. None of our kits are species specific.

Do you offer free sample kits or discounts for testing?

Unfortunately, at this time we do not offer free sample kits. Customers must purchase assay kits for their own testing, on occasion we will offer discounts.

Does the histamine kit suitable for biological samples such as blood, urine, etc.?

Since most of the biological samples contain histamine under the kit detection limit, this kit is only suitable for food, beverage, and agricultural products.

How do I prepare the solid & liquid samples?

Fish or other solid samples: Weigh ˜1 g sample and grind to paste. Weigh ˜200mg paste sample and homogenize in 800 μL 0.05% acetic acid. May vortex or use handheld homogenzier to homogenize sample. If using blender to homogenize sample, just simply scale up the sample size and solvent in 1mg: 4 μL ratio (ex: 5 g samples in 20 mL acid). Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.

Cheese: Weigh ˜200 mg sample and homogenzie in 800 μL 0.05% acetic acid. Heat in heat bath at 60°C for 10mins. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube.

Liquid samples: Fish sauce or soy sauce: 1:5 dilution in H₂O. Red wine or other alcoholic beverage: No dilution necessary.

Can I store unused reagents for future use?

Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided.

Do I need to use a standard or standard curve with each assay run?

Yes, it is highly recommended to run for each assay a standard curve, or where applicable, internal standard.

Dwidar, M., & Yokobayashi, Y. (2019). Development of a histamine aptasensor for food safety monitoring. Scientific Reports, 9(1), 16659. Assay: Histamine in tuna meat.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.