EnzyChrom™ Hexokinase Assay Kit

Protocol SDS

Application

• Hexokinase activity determination in biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue and culture media.)

Key Features

• Fast and sensitive. Linear detection range (20 μL sample): 0.22 to 100 U/L for 15 min reaction.
• Convenient. Homogeneous “mix-incubate-measure” type assay.
• High-throughput. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological samples (e.g. microorganisms, plasma, serum, cultured cells, tissue, and culture media.)

Species

• All

Procedures

• 15 min

Size

• 100 tests in 96-well plate

Detection Limit

• 0.22 U/L

Shelf Life

• 6 months

More Details

HEXOKINASE (E.C. 2.7.1.1) is a mitochondrial and cytosolic enzyme and the first committed step in the glycolysis pathway. Hexose sugars, mainly glucose, are phosphorylated using ATP to form glucose-6-phosphate. Hexokinases are implicated in a myriad of human diseases, including cancer and diabetes. BioAssay Systems’ non-radioactive, colorimetric Hexokinase assay involves a stepwise reaction based on the reduction of the tetrazolium salt MTT in an NADPH-coupled enzymatic reaction to a colored product which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

Does this kit supply hexokinase?

No, researchers must supply their own hexokinase enzyme specific to their needs.

Which Hexokinase was used to develop this kit?

A specific isozyme of hexokinase was not used, so the kit should work with any hexokinase. Glucokinase (Hexokinase IV) may require some adjustments as its kinetic parameters are very different from the other isoforms of hexokinase.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.