Typically if you are within ± 20 nanometers the assay will still work, but have slightly reduced sensitivity. Some assays have a narrower peak than others, call our tech support for a spectrum to verify.
The shelf life is 6 months from the date you received the kit. The reagents are ready to use. No reagent reconstitution is needed.
Yes, this assay works in all mammalian culture cells. We have not tested this kit in other cells, such as bacteria, yeast cells etc.
One method to normalize the results is to divide the glucose uptake activity against total number of cells or total cellular protein in the assay well. This would require a protein determination kit (e.g. BioAssay Systems’ QCPR-500 or QFPR-200).
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided.
Yes, it is highly recommended.
Liu, Meihan, et al (2019). Activation of AMPK by metformin promotes renal cancer cell proliferation under glucose deprivation through its interaction with PKM2. International Journal of Biological Sciences 15.3: 617-627. Assay: Glucose uptake in human cells.
Park, Seong-Hoon, et al(2016). SIRT2-mediated deacetylation and tetramerization of pyruvate kinase directs glycolysis and tumor growth. Cancer research 76.13: 3802-3812. Assay: Glucose uptake in human cells.
Tunduguru, Ragadeepthi (2016). Regulation of skeletal muscle insulin sensitivity by PAK1. Diss. Assay: Glucose uptake in rat cells.
To find more recent publications, please click here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to request assay service.
For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.
Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
Technical support provided by the very scientists that develop the assays.
Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.
Because we develop and manufacture the products, our prices are lower than competitors on the market
With over 200 different products, acquire all your assay kit needs in one order.
Products used by clients worldwide with distributors in over 60 countries.
BioAssay Systems is committed to producing innovative, high-quality and cost-effective products and to providing expert technical service to our valued customers.