QuantiFluo™ Glutaminase Assay Kit

Protocol SDS

Application

• For quantitative determination of glutaminase activity in biological samples.

Key Features

• Fast and Safe. Assay can be completed within 40 minutes. Non-radioactive assay.
• Sensitive and accurate. Linear detection range is 0.66-500 U/L glutaminase in a 96-well plate assay.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.

Method

• FL415/475 nm

Samples

• Biological Samples, Purified Enzyme

Species

• All

Procedures

• 50 min

Size

• 100 tests in 96well plate

Detection Limit

• 0.66 U/L

Shelf Life

• 6 months

More Details

GLUTAMINASE (EC 3.5.1.2) is a key enzyme in the metabolism of glutamine. Glutamine is an important amino acid in the metabolism of cancer cells, and increased glutaminolysis has been linked to cancer. BioAssay Systems’ DGLN-100 Kit provides a convenient fluorimetric method to measure glutaminase activity in biological samples. In this assay, o-phthalaldehyde reacts with liberated ammonia where the increase in fluorescence at λex/em = 415/475 nm is directly proportional to enzyme activity.

Can the kit?s sensitivity be increased?

Yes, you can increase the enzyme incubation step from 30 minutes to a time that provides detectable activity. The incubation time with the detection reagent will remain the same (20 minutes). Be sure to adjust the incubation time included in the calculation to represent the time you actually used.

Can I use this kit to determine the K_m of my Glutaminase?

Yes, you can use this kit to determine the enzymatic parameters of your Glutaminase. The kit comes with a 20 mM Stock solution of the Glutamine substrate. You can use different dilutions of the substrate to generate the enzymatic parameters of your Glutaminase.

Does your kit include Glutaminase enzyme?

Our kit does not include enzyme. The customer needs to provide their enzyme of choice for this assay.

For more detailed product information and questions, please feel free to email us or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.