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QuantiFluo™ Asparaginase Assay Kit



Application

  • For quantitative determination of asparaginase activity in biological samples.

Key Features

  • Safe and fast. Non-radioactive assay. Assay can be completed within 40 minutes.
  • Sensitive and accurate. Linear detection range 1.1 – 300 U/L asparaginase in a 96-well plate assay.
  • Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.

Method

  • FL415/475 nm

Samples

  • Serum, Plasma, urine, and other biological samples

Species

  • All

Procedures

  • 40 min

Size

  • 100 tests

Detection Limit

  • 1.1 U/L

Shelf Life

  • 6 months

More Details

  • ASPARAGINASE (EC 3.5.1.1) is a key enzyme in the metabolism of asparagine. Its principal use is as treatment for acute lymphoblastic leukemia and lymphoblastic lymphoma where the asparagine is an essential amino acid. BioAssay Systems’ DASN-100 Kit provides a convenient fluorimetric method to measure asparaginase activity in biological samples. In this assay, o-phthalaldehyde reacts with liberated ammonia where the increase in fluorescence at λex/em = 415/475 nm is directly proportional to enzyme activity.

Can the kit’s sensitivity be increased?

Yes, you can increase the enzyme incubation step from 20 minutes to a time that provides detectable activity. The incubation time with the detection reagent will remain the same (20 minutes). Be sure to adjust the incubation time included in the calculation to represent the time you actually used.

Can I use this kit to determine the Km of my Asparaginase?

Yes, you can use this kit to determine the enzymatic parameters of your Asparaginase. The kit comes with a 20 mM Stock solution of the Asparagine substrate. You can use different dilutions of the substrate to generate the enzymatic parameters of your Asparaginase.

Does your kit include Asparaginase enzyme?

Our kit does not include enzyme. The customer needs to provide their enzyme of choice for this assay.



For more detailed product information and questions, please feel free to email us or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to General Questions and Troubleshooting.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.

$339.00

For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.

Why BioAssay Systems?

Technical support provided by the very scientists that develop the assays.

Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Because we develop and manufacture the products, our prices are lower than competitors on the market

With over 200 different products, acquire all your assay kit needs in one order.

Products used by clients worldwide with distributors in over 60 countries.