QuantiFluo™ Ammonia Assay Kit II

Protocol SDS

Application

• For quantitative determination of ammonia and ammonium in biological samples.

Key Features

• Fast and sensitive. Linear detection range of 0.005 – 2mM ammonia.
• Convenient and high-throughput. A single component reagent. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• FL415/475 nm

Samples

• Serum, Plasma, urine, and other biological samples

Species

• All

Procedures

• 20 min

Size

• 100 tests

Detection Limit

• 5.3 µM

Shelf Life

• 6 months

More Details

AMMONIA (NH₃) or its ion form ammonium (NH₄⁺) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH₃ are very desirable. BioAssay Systems’ updated ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 415/475nm) is proportional to the ammonia concentration in the sample.

For more detailed product information and questions, please feel free to email us or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.