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QuantiFluo™ Ammonia Assay Kit II


  • For quantitative determination of ammonia and ammonium in biological samples.

Key Features

  • Fast and sensitive. Linear detection range of 0.005 – 2 mM ammonia.
  • Convenient and high-throughput. A single component reagent. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.


  • FL415/475 nm


  • Serum, Plasma, urine, and other biological samples


  • All


  • 20 min


  • 100 tests

Detection Limit

  • 5.3 μM

Shelf Life

  • 6 months

More Details

  • AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable. BioAssay Systems’ updated ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 415/475nm) is proportional to the ammonia concentration in the sample.

Do primary amine-containing buffers and chemicals interfere with the detection of NH3?

Buffers and chemicals such as Tris, Hepes, MOPS, EDTA, and various Amino Acids have been tested with this reagent. Their concentrations should be kept below 10 mM, but if they can’t be avoided, make certain to include a blank sample with just the chemical.

Do I need to dilute serum, plasma, or urine before testing?

Yes, serum and plasma should be diluted at least 10:1 with water prior to testing. Urine should be diluted 50:1 with water prior to assay.

For more detailed product information and questions, please feel free to email us or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to General Questions and Troubleshooting.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.


For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.

Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.

Why BioAssay Systems?

Technical support provided by the very scientists that develop the assays.

Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Because we develop and manufacture the products, our prices are lower than competitors on the market

With over 200 different products, acquire all your assay kit needs in one order.

Products used by clients worldwide with distributors in over 60 countries.