For quantitative determination of ammonia and ammonium in biological samples.
Fast and sensitive. Linear detection range of 0.005 – 2mM ammonia.
Convenient and high-throughput. A single component reagent. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Serum, Plasma, urine, and other biological samples
AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable. BioAssay Systems’ updated ammonia/ammonium assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 415/475nm) is proportional to the ammonia concentration in the sample.