QuantiChrom™ G6PDH Inhibitor Screening Kit

Protocol SDS

Application

• For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators.

Key Features

• Safe. Non-radioactive assay.
• High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system.
• Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed.

Method

• OD565nm

Samples

• Compounds that affect glucose-6-phosphate dehydrogenase activity

Species

• All

Procedure

• 30 min

Size

• 100 tests (Custom bulk sizes available upon request)

Shelf Life

• 6 months

Screening Services

• BioAssay Systems offers comprehensive GLucose-6-Phosphate Dehydrogenase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service.

More Details

GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in the survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics. BioAssay System’s non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.