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QuantiChrom™ G6PDH Inhibitor Screening Kit
Application
- For evaluation and high-throughput screen (HTS) of glucose-6-phosphate dehydrogenase modulators.
Key Features
- Safe. Non-radioactive assay.
- High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system.
- Rapid and reliable. Can be completed in 30 minutes and no 37°C heater is needed.
Method
- OD565nm
Samples
- Compounds that affect glucose-6-phosphate dehydrogenase activity
Species
- All
Procedure
- 30 min
Size
- 100 tests
Shelf Life
- 6 months
More Details
GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. Recently, studies have found G6PDH plays a critical role in the survival, proliferation, and metastasis of cancer cells. Therefore, inhibitors of the enzyme are attractive candidates for new cancer therapeutics. BioAssay System’s non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity. The percent inhibition of a test compound can be determined by comparing the activity of G6PDH treated with a test compound to the activity of untreated G6PDH.Does the kit come with the G6PDH enzymes or control inhibitors?
No, these are not included.
How do I store the kit?
Store all components at -20°C for long term storage.
What species of G6PDH was the kit optimized for?
The kit was optimized for L. Mesenteroides G6PDH.
What if I am interested in testing inhibition of a species besides L. Mesenteroides?
We recommend that you experimentally determine the Km and then adjust the volume of substrate so that the final concentration of the substrate in the 100 µL Reaction Volume is near the Km.
What G6PDH inhibitor was tested using this kit?
Zn(NO3)2 in dH20.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.
– Fast turnaround
– Quality data
– Low cost
Please email or call 1-510-782-9988 x 2 to discuss your projects.
$449.00
For bulk quote or custom reagents, please email or call 1-510-782-9988 x 1.
Orders are shipped the same day if placed by 2pm PST
Shipping: RT
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt
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