EnzyChrom™ Fumarase Assay Kit

Protocol SDS

Application

• For quantitative determination of fumarase enzyme activity.

Key Features

• Fast and sensitive. Linear detection range: 0.4 to 70 U/L for 30 min reaction at 37°C.
• Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD565nm

Samples

• Biological samples (e.g. plasma, serum, erythrocytes, tissue, and culture media)

Species

• All

Procedure

• 40 min

Size

• 100 tests

Detection Limit

• 0.4 U/L

Shelf Life

• 6 months

More Details

FUMARASE (or fumarate hydratase) (EC 4.2.1.2) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc. BioAssay Systems’ non-radioactive, colorimetric fumarase assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.