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EnzyFluo™ Farnesyltransferase Activity Assay Kit


  • For quantitative determination of farnesyltransferase enzyme activity in biological samples.

Key Features

  • Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
  • Sensitive and accurate. Linear detection range 0.024 – 3.2 U/L FTase in a 96-well plate assay.
  • High-throughput. Can be readily automated to assay thousands of samples per day.


  • FL340/550 nm


  • Enzyme


  • All


  • 1 hr


  • 400 tests in 384well plate

Detection Limit

  • 0.024 U/L

Shelf Life

  • 6 months

More Details

  • FARNESYLTRANSFERASE (FTase, EC catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).

I only have a spectrophotometer that reads OD. Would it be possible to run this assay colorimetrically?

One of the main substrates in this assay is a dansyl peptide fluorophore so the assay must be run fluorometrically

Does your EFTS-100 kit include a standard or calibrator?

We do not include a standard or calibrator. 4.3 is however provided as a conversion factor for determining maximum sample fluorescence. From there, activity can be calculated.

Does the kit include FTase enzyme?

This kit does not include enzyme. The purpose of this assay is to determine the FTase activity of the sample. If a pure enzyme is desired, the customer can purchase it from e.g Jena Biosciences, cat# PR-102.

For more detailed product information and questions, please feel free to email us or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to General Questions and Troubleshooting.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.

EnzyFluo™ Farnesyltransferase Activity Assay Kit


For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.

Orders are shipped the same day if placed by 2pm PST
Shipping: Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt.

Why BioAssay Systems?

Technical support provided by the very scientists that develop the assays.

Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Because we develop and manufacture the products, our prices are lower than competitors on the market

With over 200 different products, acquire all your assay kit needs in one order.

Products used by clients worldwide with distributors in over 60 countries.