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EnzyFluo™ Farnesyltransferase Activity Assay Kit



Application

  • For quantitative determination of FTase enzyme activity in biological samples.

Key Features

  • Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
  • Sensitive and accurate. Linear detection range 0.024 – 3.2 U/L FTase in a 384-well plate assay.
  • High-throughput. Can be readily automated to assay thousands of samples per day.

Method

  • FL340/550 nm

Samples

  • Biological Samples, Purified Enzyme

Species

  • All

Procedures

  • 60 min

Size

  • 400 tests in 384well plate

Detection Limit

  • 0.024 U/L

Shelf Life

  • 6 months

More Details

FARNESYLTRANSFERASE (FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).

Weight 1 lbs
Dimensions 9 × 6 × 5 in

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