Protocol SDS

Application

• For quantitative determination of FTase enzyme activity in biological samples.

Key Features

• Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
• Sensitive and accurate. Linear detection range 0.024 – 3.2 U/L FTase in a 384-well plate assay.
• High-throughput. Can be readily automated to assay thousands of samples per day.

Method

• FL340/550 nm

Samples

• Biological Samples, Purified Enzyme

Species

• All

Procedures

• 60 min

Size

• 400 tests in 384well plate

Detection Limit

• 0.024 U/L

Shelf Life

• 6 months

More Details

FARNESYLTRANSFERASE (FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).