Application
- For quantitative determination of FTase enzyme activity in biological samples.
Key Features
- Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
- Sensitive and accurate. Linear detection range 0.024 – 3.2 U/L FTase in a 384-well plate assay.
- High-throughput. Can be readily automated to assay thousands of samples per day.
Method
- FL340/550 nm
Samples
- Biological Samples, Purified Enzyme
Species
- All
Procedures
- 60 min
Size
- 400 tests in 384well plate
Detection Limit
- 0.024 U/L
Shelf Life
- 6 months
More Details
FARNESYLTRANSFERASE (FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).
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