QuantiFluo™ Urokinase Assay Kit

Protocol SDS

Application

• For quantitative determination of urokinase activity determination in biological samples.

Key Features

• Safe. Non-radioactive assay.
• Fast. Assay is completed within a 15 minute reaction time.
• Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.

Method

• FL380/450nm

Samples

• Biological Samples (e.g. Urine and serum)

Species

• All

Procedure

• 15min

Size

• 100 tests

Detection Limit

• 0.04 U/L

Shelf Life

• 12 months

More Details

UROKINASE PLASMINOGEN ACTIVATOR (urokinase, uPA) is a key serine protease involved in the degradation of the extracellular matrix that catalyzes the conversion of plasminogen to active plasmin. It acts as a thrombolytic agent to break up blood clots and when over-expressed, has been reported to influence the growth of certain malignant tumors (breast, prostate, etc). BioAssay Systems’ DUKN-100 Kit provides a convenient fluorimetric method to measure urokinase activity in biological samples. In this assay, the fluorimetric substrate reacts with urokinase so that the increase in fluorescence at λex/em = 380/450 nm is directly proportional to enzyme activity.

Does the kit come with Urokinase enzyme?

No, the user needs to provide their own purified enzyme.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.