EnzyChrom™ Glucose Oxidase Assay Kit

Protocol SDS

Application

• For quantitative determination of glucose oxidase activity and evaluation of drug effects on its metabolism.

Key Features

• Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate for 20-minute incubation at 25°C: 0.02 to 10 U/L glucose oxidase for colorimetric assays and 0.002 to 1.5 U/L for fluorimetric assays.
• Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method

• OD570nm, FL530/585nm

Samples

• Cell/tissue lysate, cell culture media, etc

Species

• All

Procedure

• 20 min

Size

• 100 tests

Detection Limit

• 0.02 (OD), 0.002U/L (FL)

Shelf Life

• 6 months

More Details

Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients. Simple, direct, and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery. BioAssay Systems glucose oxidase assay kit uses a single Working Reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to Technical Support.

Allen, R., Rittmann, B. E., & Curtiss, R. (2019). Axenic Biofilm Formation and Aggregation by Synechocystis sp. Strain PCC 6803 Are Induced by Changes in Nutrient Concentration and Require Cell Surface Structures. Appl. Environ. Microbiol., 85(7), e02192-18. Assay: Glucose oxidase in cyanobacteria cells.

To find more recent publications, please click here.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.