QuantiChrom™ DPPH Antioxidant Capacity Assay Kit

Protocol SDS

Application

• For quantitative determination of antioxidant capacity in food, beverages and biological samples.

Key Features

• Sensitive and accurate. Detects 11 μM to 300 μM Trolox equivalents in 96-well plate.
• Stable reagent. DPPH in this kit is included as a powder form that can be aliquoted to test as many or few wells as desired, or repeated testing over separate days, or weeks.
• Simple and high-throughput. This room temperature, homogenous, “mix-incubate-measure” assay can be run in as little as 10 minutes. Can be readily automated to assay thousands of samples per day.

Method

• OD517nm

Samples

• Food, beverages, and biological samples

Species

• All

Procedures

• 10 min

Size

• 120 tests in 96-well plate

Detection Limit

• 11 μM

Shelf Life

• 6 months

More Details

An ANTIOXIDANT is a molecule capable of slowing or preventing the oxidation of other molecules. Antioxidants, such as the small molecules glutathione and vitamins or the macromolecules catalase and glutathione peroxidase, protect the cells from damage by reactive oxygen species. Antioxidants are widely used as dietary supplements and in industry as preservatives in food, cosmetics, rubber and gasoline. Simple, direct and high-throughput assays for antioxidant capacity find wide applications in research, food industry and drug discovery. BioAssay Systems’ improved antioxidant capacity assay is based on the reduction of DPPH by antioxidant molecules in a sample, which results in a drop in absorbance at 517 nm. The antioxidant capacity can be quantified and expressed using a Trolox standard.

What units should I use to represent my results?

The default units for representing antioxidant capacity in DAOC-120 are trolox equivalent antioxidant capacity(TEAC) expressed in terms of μM trolox equivalents. This is calculated by plotting the delta OD for samples on the trolox standard curve and determining TEAC for your samples. Antioxidant capacity can also be expressed in terms of % inhibition of the DPPH OD517, where % inhibition = 100 x ((OD_STD0 – OD_sample – OD_{sample blank}) / OD_STD0). Antioxidant capacity can also be reported in terms of EC50, where you test several different dilutions of your sample and create a sample dilution curve. Then, using the dilution curve you can calculate the amount of sample that causes 50% DPPH inhibition.

Can I read at wavelengths other than 517 nm?

We recommend reading at 517 nm because it is the most sensitive wavelength for this assay, but you could read anywhere from 500 to 580 nm, possibly with reduced sensitivity.

For more detailed product information and questions, please feel free to email or call us at 1-510-782-9988 x 2. For more general information regarding our assays, please refer to Technical Support.

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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to discuss your projects.