Shopping Cart

EnzyFluo™ Human/Mouse AKT1(S473) Phosphorylation ELISA Kit



Application

  • For determination of AKT1(S473) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc.

Key Features

  • New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).
  • Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary.
  • Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Method

  • Cell-Based ELISA (FL530/585 nm, 360/450 nm)

Samples

  • Cells

Species

  • human, rat and mouse

Procedure

  • Assay takes 6.5 hrs, hands-on time 2.5 hrs

Size

  • 100 tests

Shelf Life

  • 6 months

More Details

  • PROTEIN PHOSPHORYLATION plays key roles in regulating protein activity within cells. A large number of protein kinases and phosphatases are known to be involved in a variety of signal transduction pathways. A rapid assay capable of monitoring such signal transduction pathways, target protein phosphorylation status and drug effects is highly desirable in life science research and drug discovery.AKT1 is a serine-threonine protein kinase that is activated through phosphatidylinositol 3-kinase by platelet-derived growth factor. In the developing nervous system AKT is a critical mediator of growth factorinduced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the AKT1. BioAssay Systems’ AKT1(S473) Phosphorylation ELISA Kit provides a rapid and sensitive assay for measuring the phosphorylated protein pAKT1(S473) in cultured cells and normalizes the level of phosphorylation to the total protein content in the same well. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study the underlying signal pathway and effects of inhibitors, siRNA or activators on the pathway. In this assay, cells grown in a 96-well plate are fixed and permeabilized. The phosphorylation of AKT1(S473) is measured using a specific primary antibody followed by a HRP-conjugated secondary antibody with a fluorogenic substrate, and a fluorescent reagent that measures the total protein in the same well.

    Haven’t Found the ELISA Kit for Your Target


    We offer two DIY kits: EFC1M-100 for mouse antibody and EFC2R-100 for rabbit antibody. All that is required is a primary antibody. If you prefer, we can develop the assay for you. Please email or call 1-510-782-9988 x 2 to request assay service.

No frequently asked questions for this new product. Please check back later.

For more detailed product information and questions, please feel free to email us. Or for more general information regarding our assays, please refer to our General Questions

No citations for this new product. Please check back later. You may click here to check if citations are available, but are not listed here yet.

If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.

– Fast turnaround
– Quality data
– Low cost

Please email or call 1-510-782-9988 x 2 to request assay service.

$509.00

For orders of 10 or more kits, please call +1-510-782-9988×1 or email us for best pricing and/or bulk order.

Orders are shipped the same day if placed by 2pm PST
Shipping: On Ice
Carrier: Fedex
Delivery: 1-2 days (US), 3-6 days (Intl)
Storage: -20°C upon receipt

Why BioAssay Systems?

Technical support provided by the very scientists that develop the assays.

Products are extensively tested and validated prior to release so researchers need little-to-no time for assay optimization.

Because we develop and manufacture the products, our prices are lower than competitors on the market

With over 200 different products, acquire all your assay kit needs in one order.

Products used by clients worldwide with distributors in over 60 countries.